Transform The Vgb Gene To Callus Of Lavandu-la Angustifolia And Study On The Waterlogged Of Transformation Body

The main objective of this study was to establish high-level somatic embryogenesis and plant regeneration of the English lavender, then transform Agrobacterium tumefaciens Vgb gene and obtain water-logging tolerance plant regeneration of English Lavender. We used to1. The MS +2,4D0.10 mg/L + KT0.30 mg/L medium groups were better than the other group. 2 to 3 times following the generation of callus in MS +6-BA2.0 mg/L + NAA0.50 mg/L culture medium continues to raise, 30 days forms massively grows thickly the bud. After waits buds 1-2cm to put in MS+6-BA1.0 mg/L to expand in the numerous culture medium to expand the numerous 1 to 2 times generations. The single bud culture medium carries on in 1/2MS+IAA on the 1.0mg/L takes root to raise, takes root rate to reach 93.33%and developed into whole plants.2. Agrobacterium-mediated callus lavender, Vgb Transgenic gene transgenic plants. Conversion conditions are as follows: Callus without pretreatment, directly in the OD value of 0.4-0.8 in the bacteria after 15min immersion dark 24h. Disposed of, it will callus and transferred to 30mg/L kan add 300mg/L Cefotaxime medium adriamycin-induced differentiation of transgenic callus, plant regeneration was normal. Step-by-step through the PCR detection screening transgenic plants, the plants were transplanted, the survival rate of more than 100%.3. Of transgenic and non-genetically modified lavender lavender for peroxidase, chlorophyll content, proline content, soluble sugar, soluble protein comparative physiological and biochemical indexes. Lavender in the oxides of genetically modified enzymes, chlorophyll content, proline content, soluble sugar, soluble protein content than non-transgenic high. Through these five indicators can be the difference between physical and non- waterlogging resistanee material indirect evidence of the expression of genes. Thus can distinguish through these five physiological target bears the waterlogging and does not bear the waterlogged material. Bore the waterlogged experiment indirect proof to bear the waterlogged gene to arrive the expression.4. The flooding experiment indirect proof has borne the waterlogged gene to obtain the expression.