Effects Of Adding Acrylate To Diet On Digestion, Metabolism And Serum Biochemical Indexes Of Sheep

The effects of methylene blue (MB), malate, acrylate were studied on gas production and DM degradation in vitro. The influence of adding acrylate to diet on digestion metabolism and serum biochemical indexes of sheep were further investigated to study the effects of adding acrylate to diet on digestion metabolism and growth of sheep.Experiment I 1g of corn straw and 0.25g of starch were added as substrate to incubation bottles in vitro, then additives of MB, malate, arcylate were added, respectively. Gas production was measured at 1, 2, 4, 8, 10, 12, 16, 20, 24h. The results showed that MB and acrylate markedly inhibited total gas production (P>0.05), whereas malate significantly increased (P<0.05). Decrease in DM degradation was observed and gas production was positive correlation with DM degradation when acrylate was added.Experiment II To study the effects of acrylte supplementation on digestion, metabolism and body weight gain of sheep, twenty-five healthy China Merino sheep (Xinjiang type) with 32kg of average body weight were selected. The sheep were fed diet consisting of 25% concentrate and 75% forage. These animals were divided into five groups, the control group, propionic groups (received 8mmol·d-1·sheep-1, 12mmol·d-1·sheep-1 of propionic, respectively) and acrylate groups (received 8mmol·d-1·sheep-1, 12mmol·d-1·sheep-1 of acrylate, respectively). The results showed that acrylate supplementation had no effect on DMI compared with the control and the propionic groups. Addition of acrylate increased (P>0.05) hemicellulose digestibility by 5.15%–9.7% and increased cellulose digestibility by 3.64%–5.73% (P>0.05). The digestibility of calcium and phosphorus in 8mmol acrylate sheep was higher 2.86% and 8.39% than that in 8mmol propionic sheep respectively (P>0.05). Compared with the control, addition of acrylate at levels of 8 and 12mmol decreased urea nitrogen excretion by 12% and 18% (P>0.05), increased nitrogen retention rate by 5.35% and 3.91% (P>0.05), respectively. Adding acrylate at levels of 8mmol, 12mmol had no significant effects on body weight gain and feed efficiency compared with the control and the propionic groups.Experiment III Blood samples were collected from the sheep jugular vein at the end of experiment II and serum were prepared. Total protein, albumin, globulin, urea, cholesterol, glucose, triglyceride, high density lipoprotein cholesterol, low density lipoprotein, glutamic pyruvic transaminase, glutamic oxalacetic transaminase, alkaline phosphatase, total calcium, total phosphorus were determined in serum . The results indicated that addition of acrylate to diet did not influence concentration of urea, calcium, phosphorus, glucose, triglyceride and glutamic pyruvic transaminase in sheep serum.This study indicated that MB, acrylate obviously suppress in vitro gas production. Decrease in DM degradation and gas production was positive correlation with DM degradation when acrylate was addd. Acrylate supplementation had no effects on sheep DMI and digestibility of DM, CP, OM, hemicellulose, cellulose compared with the control. Acrylate supplementation did not markedly affect metabolism of nitrogen, calcium and phosphorus, and blood serum urea, calcium, phosphorus, glucose and glutamic pyruvic transaminase.