Studies On The Cultivation And The Desiccation Tolerance Of Protocorms From Anoectochius Roxburghii (Wall.) Lindl.

The germination rate of Anoectochilus roxburghii seeds under different conditions and the effects of four factors(minemun culture medium, 6-BA,ZT,and NAA) on protocorm multiplication under orthogonal design were studied,as well as the desiccation tolerance of protocom was discussed.The results are as follows:1.Pollination type was an important factor on A.roxburghii seeds' non-symbiotic germination,germination ratio of the xenogamy seeds reached 78.53%,and the germination ratio of the geitonogamy seeds reached 69.62%,while the germination ratio of self-pollination was only 39.87%;the most suitable maturity of capsule appeared when it was 150 days old,the germination rate of such seeds could reach 78.59%; refrigerated storage affected the vitality of the seeds:the germination rate of the seeds was decreased with the increase of seeds' refrigerated storage time;compared with the seeds treated by sodium hypochlorite solution there was no obvious difference on germination rate;2.Variance analysis showed that NAA has a strong effect on the multiplication of protocorm and that the optimal medium for protocorm multiplication is 1/2MS+ZT0.5mg/L+NAA1.0mg/L.3.The content of soluable protein and heat-stable protein of protocorm took on the change of "M" type.After SDS-PAGE,compared with the control,a novel protein with about 28.6kDa was expressed when the protocorm was treated in 30%PEG-6000 for 2ds,3ds,4ds,5ds and in 40%PEG-6000 for 1ds,2ds,3ds,4ds,5ds.The novel protein was heat-stable.