Research On Transformation Of Chinese Wildrye With CMO Gene

Leymus chinensis is a important grass of the Gramineae family.It wide grows at the northern part grassland of our country,its value of nutrition is very high and it can grow very well in the saline-alkaline field.There is important economical value.Leymus chinensis can stand up cold,drought,wet,trample,poor soil and some saline-alkaline stress,it's adapt is very wide.But because of the grassland has been excess exploited,it makes the grassland obsolete.In order to protect resource of Leymus chinensis,improve the productivity of grassland,it is necessary to seek after the technology and approach of melioration.It is an urgently problem to how to utilize the large area saline-alkaline field.Leymus chinensis is the ideal species of grass to build meadow and control the saline-alkaline field. Although Leymus chinensis can endure saline-alkaline stress,it can't effective meliorate the saline-alkaline field.According to law of reproductive and characteristic of biology,we can plant and approve Leymus chinensis.We should utilize the capacity of bear saline-alkaline,breed some new kinds of species enduring saline-alkaline stress,it is ultimate route to control saline-alkaline.It is extremely slow to select and breed species which bear salt for traditional methods.with the development of molecular biology,people can use technology of gene engineering to improve enduring of saline-alkaline.the most efficient and ultimate method is to utilize saline-alkaline resisted gene so as to breed high-resisted species.In this experiment,we used the callis of Leymus chinensis as receptor materials.Studies have been done on genetic transformation of CMO.,The sensitive characteristic on saline-alkaline of Leymus chinensis calli and Leymus chinensis seedling was studied.We select calli and seedling which gene have transferred by the limit concentration of saline-alkaline liquor.Testing the physiological index of Leymus chinensis calli and seedling and evaluate the ability of resisting saline-alkaline of Leymus chinensis which transferred CMO gene. The results of study were acquired as follows:1.Transform the gene CMO into Leymus chinensis via particle gun,then obtain transferred gene adult plant.we examined the transformed calli after they were differentiated.That indicated that the genes had been transferred into the genome of Leymus chinensis.2.The sensitive characteristic on saline-alkaline of Leymus chinensis calliAccording the state and weight index of calli,4mmol/L NaHCO3-Na2CO3 is the limit concentration condition of calli selection.3.The tolerance intensity of Leymus chinensis transgene with transgene CMOThe max tolerance intensity of common Leymus chinensis seedling is 150mmoL/L NaHCO3-Na2CO3, contrast,the transgene transgene seedlings are grown well at 200.So it is explain that the tolerance intensity of transgene seedling has raise.4.Has carried on the appraisal to the seedling salt endurance associated physical targetThe indexes consist of content of betaine,content of proline,content of MDA,activity of SOD and activity of POD.To contrast the indexes of transformed to untransformed Leymus chinensis with stress of saltness 50mmol/L,we discovered that the content of betaine,content of proline of transformed Leymus chinensis are apparently higher and the activity of SOD and POD of transformed Leymus chinensis are higher than the untransformed Leymus chinensis too.But the content of MDA is lower.