Primary Study On Cell Suspension Culture And Dynamic Accumulation Of Secondary Metabolites Of Rheum Tanguticum Maxim. Ex Balf.

This paper reported the study of Rheum tanguticum Maxim.ex Balf.With the cotyledon of Rheum tanguticum Maxim.ex Balf.callus was inducted.Suspension culture of Rheum tanguticum Maxim.ex Balf.cell was carried out in 100 ml flask.The dynamic changes of cell growth and anthraquinones accumulation were monitored.Different explants,light,phytohormones,temperature have different effects on Rheum tanguticum Maxim.ex Balf.callus induction.The optimal condition for the growth of Rheum tanguticum Maxim.ex Balf.callus was MS+2,4-D 1.0 mg/L+6-BA 1.5 mg/L+NAA 2.5 mg/L+3%sucrose.The initial pH of medium is 5.8;25℃.During 36 days cultures,the growth rate of callus was measured every three days.The curve of the growth was like "S".Multiplication rate of callus was highest when callus was cultured in the 30th day.In the course of suspension culture,the effects of rotation ratio,inoculum density, carbon sources,sucrose concentration,culture medium and pH were investigated.The optimal condition for the growth of Rheum tanguticum Maxim.ex Balf.cell was as follows:120 rpm,1 g/40ml FW,4%sucrose;the reaction was carried out in 100 ml flask with the bottle load of 40 ml;the best medium was MS+2,4-D 1.0 mg/L+6-BA 1.5 mg/L+NAA 2.5 mg/L;the initial pH of medium was 5.8;25℃.With the cotyledon of Rheum tanguticum Maxim.ex Balf.callus was induced and screened,then established the suspension cultures.The results showed that suspension cell growth curve of Rheum tanguticum Maxim.ex Balf.was like "S";the harvest time was in the 30th day;the pH of cultured mediums went down at first,then went up gradually;the anthraquinones accumulation curve in suspension cell of Rheum tanguticum Maxim.ex Balf.was also like "S";the anthraquinones content in suspension cell was highest when suspension cell were cultured in the 30th day.The effects of salicylic acid(SA) on the cell growth and anthraquinones accumulation in suspension cell of Rheum tanguticum Maxim.ex Balf.were studied. 0.1 mg/L SA and 1 mg/L SA could promote the growth of Rheum tanguticum Maxim.ex Ball.cell and the anthraquinones production.