Study On Determination Of Gatifloxacin And Albumin In Animal Urine By Spectrographic Methods

Pharmaceutcial analysis is one of the most important components in the pharmacology category.Pharmaceutcial quality directly affects the sufferers' life health and safety.Therefore,Pharmaceutcial quality must be controlled comprehensively in order to ensure its safty,reasonableness,validity when it is used.Nowadays,the trend of pharmaceutcial analysis is to determine the trace pharmaceutcial ingredient of the complex samples simply and reliably.Therefore, Spectrographic method has become one of the most essential and important methods in the determination of pharmic products due to its easy apparatus,simple procedure, high selectivity and sensitivity.With the development of animal husbandry,the variety and quantity of the livestock and poultry have increased.Correspondingly,the type and frequency of diseases in the livestock and poultry have increased greatly.Therefore,the rapid diagnosis of animal diseases has become a very important research subject.Serum albumin is the richest protein in body fluids.Protein content in body fluids has been widely measured as the basis for the diagnosis of human diseases.But the determination of protein content in animal body fluids(such as urine,etc.) and its application research in the diagnosis of animal disease are less.Simpler,more sensitive methods for the determination of Gatifloxacin(GTFX) and albumin in cattle urine are established with using spectrophotometry as research means in order to do some basic,meaningful reference work for the prevention and diagnosis of animal disease.The major contents are described as follows:1.A somewhat thorough and all-around review of the application of ultraviolet spectrophotometry,visible spectrophotometry,fluorescence spectrum and the resonance rayleigh scattering spectrum methods in the analysis of medication and protein is given in this part.2.A new method for the determination of Gatifloxacin(GTFX) by ultraviolet spectrophotometry is established:Cetyltrimethylammonium bromide(CTMAB) can increase the absorbance of GTFX at 292 nm,so the content can be calculated according to the standard curve.This method has been applied for the determination of GTFX in tablet and powder injection,and the results are very close to those obtained by the fluorimetry method reported in notes.3.A new method for the determination of GTFX by fluorescence resonance energy transfer method is proposed:It is found that the effective energy transfer can occur between Acridine orange(AO) and Rhodamine B(RB) in the Sodium dodecyl sulfate(SDS) solution containing Britton-Robinson(B-R) solution(pH=7.00),which improves the fluorescence intensity of RB.The fluorescence of AO-RB systems is quenched with the addition of GTFX.Therefore,a novel fluorescence quenching method of indirect determination for GTFX is built with the AO-RB fluorescence resonance energy transfer.This method has been applied for the determination of GTFX in tablet and powder injection.The results are very close to those obtained by the UV method reported in notes.4.A new method for the determination of proteins is developed:Based on the binding reaction of Congo Red(CR) with proteins in the presence of Octyl phenylpolyethylene glycol(OP) and B-R buffer(pH=4.35),CR combines proteins to form complexes,causing an enhance of Resonance Rayleigh Scattering(RLS) intensity at 573nm and the enhanced RLS intensity is proportional to the concentration of protein.This method is applied for the determination of protein in bovine urine and the results are very close to those obtained by the coomassie brilliant blue method.5.A new method for the determination of proteins is developed:Based on the binding reaction of Alizarin Red(AR) with proteins in the presence of Sodium dodecyl sulfonate(SLS) and B-R buffer(pH=4.00).AR combines proteins to form complexes,causing an enhance of resonance rayleigh scattering(RLS) intensity at 533nm and the enhanced RLS intensity is proportional to the concentration of protein. This method is applied for the determination of proteins in bovine urine and the results are very close to those obtained by the coomassie brilliant blue method.