Bioinformatical Studies On The Rice Transcriptom Changes In Response To Two Species Of Virus

Rice dwarf virus (RDV) and Rice stripe virus(RSV) both have made a great impact on rice growth.Whether RDV outbreaks or RSV outbreaks resulted in a considerable decrease in production.Rice dwarf virus is the member of the first family of Phytoreovirus, Reoviridae. It is the pathogen for ordinary dwarf diseases of rice and widely distributed in rice growth regions in Southeast Asian, such as Japan, Korea, Philippines and Nepal. RDV disease commonly occurred and even ran rampant in rice production areas in the south of China . It has caused great decreases to rice yields in China. And Rice stripe virus is the typical species of the first family of Tenuivirus.The researches on the molecular mechanisms of virus-host interactions indicated that a number of defense reactions were induced after viral infection. It is of profound theoretical and practical significance to reveal the expression model of the related resistance genes under the stress of virus at the transcriptional level. To adopt Both-threaded method research this thesis.Two pilot schemes were carried out respectively. Based on transcriptom profilings obtained previously in our lab, expression differences between the resistent and susceptable rice varieties in response to RSV infection and between rice responses to RDV and RSV infection were compaired using specific programs in this study. Several pathways were shown to be differentialy altered in different rice/variaty-virus combinations. Among them were those involved in the metebolitics of gibberellins. Implications of the findings and the possible involvement of gibberellins in rice-virus interactions were discussed.