| In May 2006, A unknown severe pig disease designated"high fever"diseases occurred in many pig farms and subsequently spreaded over half of China. Infected pigs were highly contagious and high mortality rates, which more than 2 million pigs were infected and 400 thousand pigs were dead. The"high fever"disease has caused tremendous economic losses to the hog industry in China. In July,The disease appeared in Fujian Province, but spreaded rapidly . The pig samples which had the "high fever" symptoms were collected from the swinery in Fujian province. The suspected viruses isolation and identification were done from the samples. RT-PCR test was used to identify PRRSV.In order to search for the proteins related to virulence factor, the proteomics of the cell proteins were compared between PRRS 071030 strain and Fuzhou strain which vaccinating in normal Marc-145 cell by two-dimensional gel electrophoresis. PRRS 071030 strain and Fuzhou strain were cultured in normal Marc-145 cell at 37℃and 5%CO2 for 72 hours and the proteins sample were extracted from cell. The 2-DE gel was conducted using the pH3-10 IPG strip for the first dimension IEF and followed by SDS-PAGE. After electrophoresis, the gels were stained and analyzed using Image Master 2DE Platinum 6.0 software. The results showed that the normal Marc-145 cell, PRRS 071030 strain and Fuzhou strain, which were cultured in normal Marc-145 cell, had 188,252 and 286 protein spots with similar molecular masses. However there were about 12 specific protein spots were identified for normal Marc-145 cell and Fuzhou strain, 2 protein spots were identified for normal Marc-145 cell and 071030 strain, only 1 protein spot were identified for Fuzhou strain, 3 protein spots were identified for 071030 strain, 9 protein spots were identified for 071030 strain and Fuzhou strain, The expression profile of those proteins were also analyzed. These results provided better understanding on pathogenic mechanisms of PRRSV at the protein level. |
PDF Full Text Request