The Investigation Of Content Of Pesticide Residue In Main Chinese Traditional Patent Medicine In Guizhou

The pesticide residue of Chinese traditional patent medicine is one of the problem that we need to be resolved when it is face of internationalization and modernization. This study is response to development needs of Modernization of Traditional Chinese Medicine of Guizhou Province, the corresponding detection methods were built by investigation on the pesticide residue of Chinese traditional patent medicine in Guizhou province. On this basis, a detection Platform of pesticide residues of Chinese traditional patent medicine in Guizhou Province was built to give the quality assurance and technical support for modern and international Chinese traditional patent medicine in Guizhou. Main content of the study are as follows :1 The organochlorine pesticides residues were determined which from different major Chinese traditional patent medicine of Guizhou Province. The results showed that there have detected 52 organochlorine pesticides of 71 sample, and the detection rate of 73. 24%, the majority of samples pesticide residues is less than 0. Olmg/kg. According to Chinese Pharmacopoeia(2005 version) organochlorine pesticide residues of the limited standards, there is no excessive samples; referencing to "Green Trade Standards of Importing & Exporting Medicinal plants & Preparations" , over standard rate is 1.4%.2 The Pyrethriods Pesticide residues were determined which from different major Chinese traditional patent medicine of Guizhou Province. To establish a method for determination Pyrethriods Pesticide in Chinese traditional patent medicine by the electron capture detector-capillary gas chromatography. The recoveries and RSD of Pyrethriods Pesticide were 78. 69%～113. 52%. The linear ranges were 0.10～5.0μg/mL It can be used to detect Pyrethriods Pesticide from Chinese traditional patent medicine. There were 25 of 71 sample was detected Pyrethriods Pesticide, and the detection rate is 35.21%. Referencing to "maximum residue limits of Tea in the European" , 71 samples of Chinese patent medicine did not exceed the standard.3 The Organophosphorous Pesticide residues were determined which from different major Chinese traditional patent medicine of Guizhou Province. To establish a method for determination Organophosphorous Pesticide in Chinese traditional patent medicine by the pulsed flame photometric detector (PFPD)- gas chromatography, and the column was OV-1701 capillary column. all the samples were not detected of organophosphorus pesticide residues. This may be related to degradation period of organophosphorus pesticide is relatively short, the properties instability. Generally speaking, Chinese traditional patent medicine did not have organophosphorus pesticide.4 To establish a method for determination Organochlorine pesticides in Chinese traditional patent medicine by -gas chromatography. The sample was extract with acetone , dichloromethane , GPC purification, and detected with electron capture detector (ECD). The recoveries of Organochlorine pesticides were 87. 21%～109. 54%. The detected limited were 0. 000182～0. 003295μg/mL. It can be used to detect Organochlorine pesticides from Chinese traditional patent medicine.