Effect Of Different Additives On Degistive Characteristics Of Ensiled Alfalfa In Vitro

Large amount of protein is degraded into Non-protein Nitrogen(NPN) in the process of alfalfa fermentation,which can not be effectively absorbed by animals.The process of protein degradation and the effectiveness of 2 silage additives,Micromanager No.9927 and Siloguard,which are used to inhabit proteolysis,were studied in this experiment.Seven different treatments were applied to newly harvested alfalfa,including a control(0 g/t),3 levels of Micromanager No.9927(11.35g/t,22.7g/t and 34.05g/t) and 3 levels of Siloguard(227 g/t,454 g/t and 908g/t).Samples were collected at different fermentation time 0,1, 2,4,7,14,21,40d.The following parameters were measured:pH,DM,CP,WSC,NPN,NH3-N,AA-N, Peptide-N and the rate of in vitro DM,CP,NAF and ADF digestion.The results showed that lactic acid bacterial inoculants in alfalfa silages significantly improved silages quality,with reduced pH,increased Total Nitrogen and reduced NPN,NH3-N and FAA-N.The activity of protein degradation enzyme was inhibited and the rate of in vitro DM,CP,NAF and ADF digestion were remarkably enhanced.Among 3 application levels,the rate of 22.7g/t lactic acid bacterial inocolants silage additive produced the best quality alfalfa silage.The effectiveness of Siloguard is not as good as lactic acid bacterial inoculants.As a whole,Siloguard played an active role in improving the quality of alfalfa silage,with reduced pH,increased Total Nitrogen, reduced NPN,NH3-N and AA-N,inhibited proteolytic enzyme activity and elevated in vitro ADF digestion rate.The utilization rate of alfalfa silage was enhanced.The application rate of 454 g/t Siloguard produced the best quality alfalfa silage.