Research Of Elicitor In American Ginseng Root

In this paper,the American ginseng root for the material,in the callus induction,the optimization of culture conditions,induced by the son of American ginseng roots with the impact of such areas were studied. Key findings are as follows:The effects of different explants,pre-training time,a total culture time,the concentration of bacteria and bacilli,AS add to the root of American ginseng-induced effects.Through experiments that:R1601 induction of Agrobacterium 1 -2 weeks Age of American ginseng leaves,stems and leaves with David Stem,David leaves to stems with the root of the highest rates of induction,pre-training 48 h,after infection.The explants cultured in a total of 48 h will get better results.Activation of the process of adding 100μmol / LAS hairy roots can increase the rate of induction.Suspension of optical density control OD₆00 = 0.6,with 20 min time for optimal conditions.In the optimization of culture conditions,respectively,with four kinds of liquid medium MS,improved White,SJ-1 and B5 culture of American ginseng roots.SJ-1 liquid most conducive to the growth of roots,on growth in multiples of up to 31.3 times that of the liquid under the conditions of the training MS 1.7 times. The total accumulation of saponin White improved to the best effect(8.10%).The root of the integrated growth and the accumulation of saponin,MS medium most beneficial.Sucrose best interests of the American Ginseng root growth and also the most conducive to the accumulation of saponin.The most appropriate of American ginseng root growth and total saponin of the pH value is 5.8.Induction of the American Ginseng root,the impact,induced by the addition of one hand can enhance the metabolic activity of certain enzymes followed by further enhancing the accumulation of metabolites of Health,on the other hand the addition of lead-induced cell activity decreased,and even cell death.When induced by excessive concentration,cell activity decreased synthesis of secondary metabolites of the impact of increased gradually,over the induction of synthesis of secondary metabolites in the promotion and therefore inhibit the accumulation of secondary metabolites.This paper studies the salicylic acid,methyl jasmonate two of the best add-induced concentration,time and the role of the two-induced synergies.Eventually come to the conclusion:the best concentrations of salicylic acid for the 0.6 mg / L,the time for the role of 144 h.Add the best of salicylic acid concentration and the role and effects of time and Miao Zhi-qi,who reported that the De-Jiang Li,who reported that Wen-chi Lan Yu Long,who reported the basic line.Most scholars believe that the concentrations of salicylic acid should not exceed 1.0 mg / L,when the acid concentration higher than 1.0 mg / L,is not conducive to plant growth and the accumulation of secondary metabolites.Add methyl jasmonate the best concentration for 10 umol / L,the time for the role of 192 h-240h.Experiment proved that methyl jasmonate can induce the American ginseng root secondary metabolites in the accumulation of ginsenoside.Methyl jasmonate of the plant to promote this role and the best concentration of Hunan,Hubei and the Soviet Union,Mei Xingguo, who reported that Chun-Rong Zhang,Li Ling's report,Liang Xu Sheng,Xiao-Feng Xue,Fu Chunxiang,etc. The reports were consistent with the basic.Most of the scholars reported when the concentration of methyl jasmonate than 20 umol / L when the cells will have a negative effect.Two induction of the best synergy of salicylic acid ratio of 0.4 mg / L,methyl jasmonate 10 umol / L.