Oxidative Damage Of Di(2-ethylhexyl)-phthalate (DEHP) To Gold Crucian Fish

Ethylhexyl phthalate ester(di-(2-ethylhexyl) phthalate,DEHP),also known as iso-octyl phthalate esters,is one of the phthalate esters(PAE).It is one of the most widely used phthalates in the plastics industry as the most important plasticizer.Because DEHP is not binded with covalent chemical bond to the plastic,it is easy to transfer from the plastic to the outside environment,resulting in the air,water and soil pollution. Research has shown that,DEHP can entered through a variety of ways in vivo,damaging many organs and tissues,lead to many diseases.In this study,gold crucian carp were used to detect the DEHP-induced the impact of the biochemical indicators of their tissues and organs.In order to carry out phthalate-induced toxic effects on gold crucian carp and to chose biomarker.The study on the protection of water environment,the establishment of Phthalic Acid Esters in biological detection indicator is of great significance.It can provide scientific basis of safety nutrition for aquatic animals.1.Gold crucian carp brain tissue and kidney tissue dynamic changes in SOD caused by DEHPIn this study,gold crucian carp was used to the experimental materials,the different doses(50mg / L,100 mg / L,200 mg / L,400 mg / L) of DEHP into the water,exposure with 14d.In this experiment,the brain tissue and kidney tissue of gold crucian carp were used for the experimental material.Determine the tissue's SOD viability with NBT.The results showed that:50mg / L of DEHP could cause gold carp brain and kidney oxidative damage,the brain tissue and kidney SOD was induced significantly(p＜0.01);SOD activity that was induced by gold crucian carp brain and kidney with a large amount of O^2-,gold crucian carp has been in the oxidative stress.When the concentration of DEHP was 200mg / L,SOD decreased clearly,and the higher the concentration,the more obvious decline,showing a clear dose - response relationship.2.Gold crucian carp brain tissue and kidney tissue dynamic Changes in MDA content caused by DEHPIn this study,gold crucian carp was used to the experimental materials,the different doses(50mg / L,100 mg / L,200 mg / L,400 mg / L) of DEHP into the water,exposure with 14d.In this experiment,the brain tissue and kidney tissue of gold crucian carp were used for the experimental material,with thiobarbituric acid(TBA) to determine lipid peroxidation product malondialdehyde(MDA) content of brain tissue and kidney in order to determine the extent of lipid peroxidation.The results showed that:With increasing concentrations of DEHP,lipid peroxidation levels were increased in the brain tissue and kidney.When the concentration of 200mg / L,the MDA content of brain tissue significantly increased(p＜0.05);the MDA content of kidney also increased clearly(p＜0.01).In lipid peroxidation,the sensitivity of kidney than the brain,and this may be related to brain tissue antioxidant mechanism.When the concentration of DEHP was 400mg / L,the brain tissue and kidney MDA content both in a significant increasement(p＜0.01).It was caused by the high concentration of DEHP gold crucian carp fat cell membrane serious injury.There are two reasons:on the one hand it is caused by the rupture of membranes,and the release of contents;on the other hand,membrane lipid peroxidation peroxide generated can also be used to enlarge the role of oxidative damage,and further extensive damage caused to the body.