The Detection Method Study Of Meat (Fish)-borne Parasites

Content: Food safety is related to the health of the people and build a harmonious society ,which is the major strategic issues, but the parasites in food (egg) pollution caused by food-borne disease epidemic, has not been given sufficient attention. Faced with the growing problem of parasites in the meat and aquatic products food safety issues, vigorous need research and develop of new, rapid, sensitive, integrated, high-throughput detection technology, has become an important direction. While the existing detection methods are limited to check and parasitic pathogen immune diagnosis technology, can only be done after the onset, but can't for prevention. Therefore, the establishment of parasites in food primarily the rapid detection method is very necessary.Clonorchiasis is the most common in the fish-borne parasite, the paper applicatded the conventional PCR, denaturing high-performance liquid chromatography technology and real-time PCR for detecting clonorchiasis, in order to build a rapid, sensitive and of specific platform for detection fish-borne parasite. use of parasites ribosomal no-transcription district ITS2 region highly variable nature of designed specific conventional PCR primers, and used primer express 3.0 software in the variable region of Clonorchis sinensis looking for real-time PCR of specific primers and probe, after experiments. That Confirmed the self-designed conventional PCR primer and real-time PCR test using primers and probe are both specificity. Real-time PCR and denaturing high-performance liquid chromatography detection of Clonorchis sinensis the sensitivity has reached 1.37×10^-5ng/μL. It also means that the establishment of gene chips used in food tapeworm, trichinosis and toxoplasmosis three kinds of meat-borne parasite rapid detection technology, and further for people suffering from a beast of tapeworm, a parasite trichinosis and toxoplasmosis infection early diagnosis and testing . Selection of Toxoplasma B1 gene, the gene trichinosis SB2, tapeworm 12 SrDNA variable region to design the primer and probe, made of glass at the point-like gene chips. DNA primer after Parasite amplified with the chip on the probe hybridization, and then by scanning images of specific results, such as sensitivity to judge, also conducted a simulation of pollution test. The results showed that: the design of primers can be amplified three parasites, and with the high specificity of the probe. In this system the sensitivity of gene chip hybridization detection about 1.5×10^-5ng/μL. as an example of trichinosis and toxoplasmosis in food, can be detected in 10 of Toxoplasma tachyzoites / 200 mg; can detected a trichinosis / 200 mg.