| Our country(?) water resources(?) are rich,Therefore,the development fresh water pearl cultivation is our country pearl industry way that must be taken.The triangle sail freshwater mussel is the most main fresh water nurtures the pearl oyster,the correlation research are many.But affects the pearl cultivation seriously the freshwater mussel seasonal contagious disease research then relative lag,At present only at seasonal contagious disease physiological pathological change,the epidemiology,virus's separation and the warning grade aspect has little reported,Must prevent and control the freshwater mussel seasonal contagious disease thoroughly,must conducts the molecular level research in the virus to the freshwater mussel morbidity correlation gene and the viral gene, Understood its pathogenesis,comes up preventing and controlling freshwater mussel seasonal contagious disease from the basis.The main body of a book the freshwater mussel coming to study the Dongting Lake area lateen startling in two aspects the venereal disease aetiology is in popular from the bacterium and virus.Bacterium aspect is that the freshwater mussel bacterial venereal disease aspect aetiology is in popular by the fact that identifying,disease freshwater mussel organization pathological mechanism studying the Dongting Lake area lateen from pathologic investigation,separation of the pathogenic bacteria being in popular venereal disease being in popular to Dongting Lake area lateen freshwater mussel mainly;In the aetiology coming to study the Dongting Lake area lateen freshwater mussel venereal disease is in popular virus aspect form,size being a mirror pass virus's separation purification and electricity mainly observe a virus etc.whether or not existence virus factor virus's form,size etc.makes a definite diagnosis with the electricity under the mirror virus's kind,such is that medicine prevention and cure lateen freshwater mussel seasonal febrile diseases provides the theory basis further;Provide the forceful guarantee for Dongting Lake area lateen freshwater mussel estate develops,kind of quality resource provides the important theory basis to good character developing and protecting the Dongting Lake area lateen freshwater mussel's rationally. Hyriopsis cumingii epidemic disease is always the resrtictive factor of our country fresn water(?) production.this article.from the two aspects of germina and virus,discusses the epidemic disease pathogen of Dongting Lake area Hyriopsis cumingii.Through to diseased Hyriopsis cumingii pathogenic microorganism's severance,purification and biochemistry identification,six kinds of bacterials,Aeromonas sobria,Pseudomonas fluorescens,Serratia odorifera,Klebsiella planticola,Klebsiella oxytoca,Burkholderia diminuta,were finded.Canrring on combat poision,drug sensitive test,Aeromonas sobria and Pseudomonas fluorescens are confirmed to be the pathogens.Simultaneously diseased Hyriopsis cumingii organization was taken to homogenize and centrifugate,transferring the clear supematant to a fresh tube,then virus suspending liquid was gained when the clear supernatant was treated by double antibiotics(penicillin,streptomycin) or filtered by microporous filtering film.After artificial infection healthy mussels were all died of the same symptom.The diseased organization was fixed by glutaraldehyde,resin embeded, negative staining,then the sphere shaped or oval shaped virus particles could be observed under the electronic microscope.The particles,diameter were 40-290nm or so,whose surface were full of tenuous prominencies,there were several compact "sand grains", diameter 20-30nm,which could be distinguished from a few of viruses.Therefore,the union epidemiology investigation,pathogenic bacteria's immersion and the injection tests as well as organizes the pathology observation and virus's electron microscope observation, may determine initially:In April,2005-July,2007 the Tungting lake lake district triangle sail freshwater mussel seasonal comagious disease primary cause of disease inlays the drilling mud cuttings virus,but warm friendly Shan Baojun,produces the alkali pseudomonad for the sequential cause of disease. |
