Analysis Of Differential Expressed Protein In Alternanthera Philoxeroides Under Drought And Salt Stress

The thorough understanding of the physiological characteristics and the molecular genetic basis of plants under drought and salt stress has become a front concent of plant molecular biology research in the recent years. The proteins that are the end product of gene expressions play important roles in the plants in response to stresses.Alternanthera philoxeroides has broad adaptability and strong resistance to various stresses. Understanding of the mechanism of Alternanthera philoxeroides's resistance to stresses, may provide the theoretic basis for further improving plant's ability of combatting drought and salt stress. In order to identify differentially expressed proteins under drought and salt stress, and further cloning related genes, the method of extracting proteins from different tissues of Alternanthera philoxeroides was established, and SDS-PAGE analysis of expressed ptoteins was carried out. The result indicated that, the expression of 42, 66, 72 kD proteins increased and the expression of 29 kD protein decreased after drought stress; the expression of 40, 66, 69 kD proteins increased and the expression of 38 kD protein decreased after satl stress. This research lays a foundation for further study on molecular mechanism of drought and salt stress induced protein regulating in Alternanthera philoxeroides.Actin gene which is usually called house-keeping gene encodes a consecutive expressing protein in vivo. Actin is commonly used in quantitive RT-PCR as internal standards. For cloning actin gene, primer was designed according to the EST of actin gene of one kind of pigweed plant in the Genbank. Total RNA was isolated from the roots of the Alternanthera philoxeroides, and RT-PCR was used to amplify the gene. A 230 bp DNA sequence was obtained. Sequence comparison with the EST indicated that it is actin gene fragment of Alternanthera philoxeroides.It could benefit to further analyse differentially expressed proteins from Alternanthera philoxeroides by two-dimensional electrophoresis, as well as to clone the related genes under drought and salt stress.