Studies On The Extraction And Purification Of Gossypol And Its Biological Activity To The Heliothis Avmigera Hubner, Verticillium Wilt

The gossypol other name gossypium's toxin, is the cotton own antibiotics, has certain resistance to many cotton plant diseases. Its free gossypol toxicity is very big, is one kind of natural insecticidal and antibacterial biological activity material. This article study the different gossypium which is relevance the gossypol contains quantity and resistance, the gossypol difference extraction ,purify the method and Its biological activity on the Heliothis avmigera hubner, verticillium wilt of cotton ,obtains the following result:1. Through gossypol determination optimal, indicated that the AOCS this law ease of operation, is accurate, is reliable, moreover the instrument is ordinarier, the serviceability to be broad.The different gossypium to Heliothis avmigera hubner resistant degree research, finally indicated that the different gossypium is between has the obvious difference, G.barbadense resistance good in G. hirsutum, drives to the Heliothis avmigera hubner to evade the nature to be good, but suppresses the Heliothis avmigera hubner growth effectively, is one kind of very good anti-insect idioplasm resources.2. Further improves to the methyl alcohol gossypol's determination method, this law has not used the toxic big aniline, the method is safer conveniently, is accurate, is fast. With 0.1% citric acid +95% methyl alcohol composition's methyl alcohol and the benzine by 45:25 proportion, compares several other methods to be good, the gossypol leaching production rate reaches 63.8%. To the best leaching's method orthogonal optimization research, indicated works as the temperature for 40℃, leaching time 35 min, the cottonseed and the resolver proportions is 1:4 (g/ ml) the gossypol leaching production rate is highest, is 69.23%. The water dilution method raises the purification acetic acid gossypol overall yield is 38.86% thickly, is not obvious with the aniline process difference. But in the water dilution method preparation process has not used the toxic substance aniline, the craft is simple, the cost is low, has certain promotion use value.3. Through the gossypol to the Heliothis avmigera hubner dose effect research, indicated that the gossypol has the obvious dose effect to the Heliothis avmigera hubner different developmental stage. The gossypol to young age Heliothis avmigera hubner LD50 is 1.939mg/g, and increases along with the gossypol dosage, the Heliothis avmigera hubner larva mortality rate increases; Larva experience time extension; The cocoon heavy reduces gradually; The prosopon amount of spawned egg reduces, the sterile egg increases, the reproductive capacity drops; Causes its population quantity to reduce very quickly. Obviously the gossypol to Heliothis avmigera hubner's nutrition metabolism, the growth and the reproduction has the inhibitory action, has well suppresses the biological activity.The gossypol to the Cotton plant bugs distribution, occurs has certain inhibitory action. The gossypol of influence is small to the natural enemy, to environmental safety.4.Is to the different gossypium of the gossypol content and the cotton anti-verticillium wilt's resistant relations preliminary study, indicated that the gossypol content and the cotton variety anti-verticillium wilt present is related. the G.barbadense gossypol material biological activity ingredient content high, disease resistance, is the high anti-verticillium wilt special idioplasm resources. The gossypol extracts to the cotton verticillium wilt disease germ has the very good bacteriostasis activeness, may prevent the hypha to grow, to cut the spore germination probability. Obviously, the gossypol is one kind of natural bacteriostasis active material.