Inhibitory Effects And Antifungal Active Substance Of Bacillus Subtilis BS-2 On Pathogen

The results of confrontation culture and anti-bacterial experiment indicated that Bacillus subtilis BS-2 had strong inhibition to the fungi Neurospora,Trichoderma and Mycogone, and could produce the volatile substance to inhibit the germination of the spores of the fungi Trichoderma. The BS-2 mutation caused by ultraviolet were nominated as BS-2-E with increasing of anti-bacterial activity and BS-2-N without it. Use LC-MS to analyze BS-2, BS-2-E, BS-2-N and the basic extracts from the fermentation liquids of fungi Neurospora BS-2 and compare the difference between the antibacterial ingredients. Initially inferred that the main materials of BS-2 are iturin and Fengycin B.Using BS-2 ferment on fungi Neurospora to take initial exploration in biolobical control is also discussed in this paper.