The Analysis Of Glutathione In Changbai Mountain Medical Plant And Edible Fungi

Glutathione is self-synthetic and endogenous active tripeptide (Z-glutamyl-Z-cysteinyl-L-glycine) in organism, it classified into reduced glutathione (GSH) and oxidized glutathione (GSSG). There are biological functions of glutathione, such as participating in absorption and transportation of amino acids, influencing the synthesis of proteins and DNA, scavenging peroxide and free radical in vivo, maintaining morphology of erythrocytes, carrying oxygen ability and detoxification, etc. Recently, the report of exogenous glutathione (mainly injection, tablet form) preventing and curing diseases gradually become increased; the application of exogenous glutathione as food additives and health products is attracting worldwide industries' attention. However, effective active function of exogenous glutathione is always common subjected to query. The main reason for the question is the worse biological compatibility of chemosynthtic glutathione. Thus, an effective measure screening of the rich and the better biological compatibility glutathione and its precursors in natural product became essential. Glutathione is widely distributed in animal and plants (including edible fungi).Based on the progress of the research of glutathione, medical plant and edible fungi (wild and cultivated) on Changbai Mountain area can be used target material to screen the glutathione. The content of GSH and GSSG was determined simultaneously by means of high performance liquid chromatography (HPLC). The results showed that the highest total content of glutathione in Schisandra chinensis (Turcz.) Baill , followed Rhodiola sachlinensis A.Bor and Panax ginseng C.A.Mey, moreover,the radio of GSH/GSSG of the wild species is significantly higher than the cultivated. Furthermore, the content of glutathione in the wild Pleurotus ostrcatus is significantly higher than Lentinus edodes, Tricholoma matsutake,Arimillaria mellea and Auricularia auricular. The radio of GSH/GSSG of the wild species is also significantly higher than the cultivated, among the radio of GSH/GSSG of the wild Auricularia auricular is the highest. In this thesis, the optimum experimental conditions for the determination of glutathione and the separations of reversed phase ion pair chromatography of the GSH and GSSG were investigated, such as method of removing protein, selection and proportion of various flowing phase, etc. In addition, the result will offer important reference data for exploitation of economic plant and fungi on Changbai Mountain area, for screening the rich glutathione of plant and fungi having medicine and food function, for discussion about studies of plant stress resistance to the radio of GSH/GSSG.