Expression Profiles Analysis Of Transcription Factor Families And Their Target Genes During Dedifferentiation Of Mature Wheat Embryos

The aim is to study the molecular mechanism of embryos dedifferentiation in mature wheat by using the common wheat cultivar Yumai 18 (Triticum aestivum L.) in Henan Province, We analyzed the expressions of genes related to transcription factor families and their target genes during dedifferentiation of mature wheat embryos at transcriptional level with a Affymetrix wheat GeneChip. The referenced calli were 0 hours'samples. Those genes were greater than 2 or shorter than 0.5, namely which exhibited a greater than two folds change in gene expression level during dedifferentiation of mature wheat embryos, were considered as meaningful different expression genes by induction of the relevant moments of the 2,4-D in 0h, 2h, 6h, 12h, 24h, 72h . The results showed that by using the Affymetrix wheat GeneChip including 61127 probe sets, which represented 55085 genes, about 15000 genes of those were happened to have meaningful expression change. For transcription factor families and their target genes of meaningful changes there is a further study in the meaningful changes. In mature embryos, the total amount of 425 genes related to those 24 types of transcription factor families were found to play a role in this process. And the number of up-regulation genes, down-regulation genes ,and up-and of down-regulation genes were 139, 232 and 54, respectively. Three types of transcription factor families and their target genes from 42 types of transcription factor families have been done a further analysis. The results were as follows:1)MYB transcription factor families and their target genes This paper mainly aimed to study the 12 transcription factor families and 27 downstream genes. According to their overall expressions on the chip, Lhcb5 was negative regulated by MYBST1, which played a role during the stage of signal transduction (SST) of the process of mature wheat embryos dedifferentiation. 4cl1 and C4h were negative regulated by MYB4-like, they played a big role during the stage of signal response (SSR) of the process of mature wheat embryos dedifferentiation. Adh1 and Rd22 were negative regulated by MYB2, they also played a big role during the stage of signal response (SSR) and stage of calli formation (SCF) of the process of mature wheat embryos dedifferentiation.2)AP2_EREBP transcription factor families and their target genes BBM and ANT of AP2 subfamilies can maintain the ability of cell division and regulate cell proliferation, organ growth and calli formation. In the wheat gene chip there were not direct description of the related genes for those two genes, but described as AP2 subfamily transcription factor genes had 10 genes. According to the physiological and biochemical characteristics of the division period of mature wheat embryos, the main period of callus formation is after 24h. Three genes were largely started at 24h in the chip, they were respectively gb:BJ277744, gb:CA651253 and gb:CK200155. According to their expression, gb:CK200155 gene may be homologous gene of BBM and ANT genes, which increased up to 79 times of the controlled sample in the dedifferentiation phase of mature wheat embryos. Its functional verification would be proved by the further method of knockout or RNA interference, and other ways in the period of callus formation.3)MADS transcription factor families and their target genes This paper mainly aimed to study the 32 MADS transcription factor families and 2 target genes. According to their overall expression in the chip, they played a big role in the stage of signal transduction (SST) and stage of calli formation (SCF) of the process of mature wheat embryos dedifferentiation. Their relevantly detailed functions have to be further studied.