Effect Of Anaerobic Digestion On Indicative Bacteria In Cow Manure And Effect Of Effective Microorganisms On Them During Anaerobic Digestion

China is one of the most important milk producing countries in the world. With the development of animal husbandry, the cattle industry has gradually evolved from a diversified to a specialized and intensified production system. The change of production system has led to difficulties in the hygienic disposal of dairy cattle manure, resulting in serious environmental pollution. Dairy cattle manure is known to harbor a certain amount of pathogenic microorganisms, especially zoonotic pathogens. These pathogenic microorganisms through contaminated water, air and soil, and caused the outbreak of epidemic diseases, endangering human and animal health.According to the requirements of environmentally hygienic disposal and resource processing , the livestock and poultry droppings dirt sound treatment must involve effective disinfection of these micro-organisms to meet the environmental and ecological protection, to protect the environment and human health and promote animal husbandry sustained and healthy development. Anaerobic digestion of excreta disposal is an effective pollution control and waste recycling use of technology for Chinese shishang aquaculture industry shortage of funds, scarcity of resources of the nation. The widespread application of effective microorganisms in the cultivation, breeding and environmental protection has systematically shown to promote plant growth, improve the animal disease-resistant ability, remove fecal stench and improve the ecological environment, and many other aspects of the role and a good application potential. According to sample survey family-size biogas plants which is constructed by the small farmers near Tai'an, preliminarily understood the effect of anaerobic digestion of cow manure on content of anaerobic bacteria and aerobic bacteria, Fecal Coliforms and fecal Streptococcus group in manure. With the findings, in laboratory conditions, effect of bioaugmenting mesophilic anaerobic digestion of dairy cattle manure with effective microorganisms(Pseudomonas, Bacillus, Lactobacillus, , Microzymes and Actinomycetes) on the digestive system stability and indicative bacteria(Fecal Coliforms, C. perfringens, the total number of anaerobic and aerobic bacteria) was evaluated.Through the survey results of effect of family-size biogas plants on indicative microorganisms during anaerobic digestion of dairy cattle manure show that cow manure digesters dealing with small, can significantly reduce the cow manure in the aerobic and anaerobic bacteria, Fecal Coliform and Fecal Streptococcus group content. However, the content of Clostridium perfringens in cow manure are not affected. Although the content of major indicative microorganisms decreased significantly in the cow manure after anaerobic digestion, the slurry output still harbor a considerable amount of the anaerobic, aerobic, Fecal Coliform and Fecal Streptococcus group, which remains high, reaching less than the national requirements of livestock Bird droppings emission standards. Therefore, it is necessary to further improve the decline of pathogen numbers during anaerobic digestion of the small cow manure digesters.Bioaugmenting mesophilic anaerobic digestion of dairy cattle manure with Pseudomonas, Bacillus, Lactobacillus, Microzymes and Actinomycetes can accelerate reduction of Fecal Coliform significantly. However, the number of aerobic and anaerobic bacteria did not change significantly during anaerobic digestion. This may be due to the large number of propagation of added micro-organisms during the latter period increase the total number of bacteria in the digestive system. Due to unsuited to the environment of the early stages, the number of Clostridium perfringens which added to the digestive systems reduces. 10 days later, because of adapt to the environment, the basic amount of Clostridium perfringens has not changed. Bioaugmenting have no impact on the removal of Clostridium perfringens during anaerobic digestion. At the acid production phase, bioaugmented effective microorganisms can increase acetic acid and butyric acid production and inhibit the formation of propionic acid at the same time, to form a stable, good anaerobic digestion system. In the process of anaerobic digestion, NH4+-N and pH can inhibit the growth of pathogenic bacteria to a certain extent, but not the major factors.