The Studying On Some Biology Characteristics And Trehalose Metabolism Of Acantholyda Posticalis

Acantholyda posticalis Matsumura(Hymenoptera: Pamphiliidae)is a major pest of defoliator to pine tree in china. For further study of biological characteristics of complementary nutrition, changes in trehalose content in haemolymph of over-wintering larva and glycogen content of over-wintering larva and trehalase activity, suggesting some integrated control measures. The main results are as follows:1. Catched adult in late April, females and males were taken at different ratio into different container. They were provided fresh pine needle and diferrent concentration of honey and sugar as their complementary nutrition. The results showed that: there showed significant difference in different treatments. Adult longevity was prolonged at concentration of 1% and 0.5%, but it was contrary at concentration of 5% and 10% when used honey;adult longevity was prolonged at concentration of 0.5%,1% and 5%, but it was contrary at concentration of 10% when used sugar.Their ovipositing increased when given 1% honey and sugar for its complementary nutrition. there showed significant difference in different treatments.2. Larvae were collected every month from June2007 to March2008 and treated as follows: First, applied hot alkaline process extraction glycogen. Second, applied anthrone method for determination of glucose including. Meanwhile, applied TLC method for separating carbohydrates, and then use UV-2201 ultraviolet spectrophotometer to estimate content. The results showed that: there has no significant difference between overwintering larvae and mature larvae. The titres of glycogen in fat body dropped gradually after into soil, lowest in December, increased up to March. The titres of haemolymph trehalose were more steady and changed little from July to October, there was no significant difference in diferrent months, but were going up in November, maximum in December, then decreased quickly in Jan and increased slowly until post-overwintering stage. Overwintering larvae were treated and used 3, 5-Dinitrosalicylic Acid to determine trehalase activity. The results showed that: Trehalase activity was very high in periods of overwintering, increased before September, then decrased to the lowest in December and increased slowly until post-overwintering stage. Trehalase activity is highest in March, more than 85.7 percent of the larva in June, increased 2.79 times than December.4. Infrared analyzer of CO2 determined respiration of larva. The result is: With larvae going into overwintering, larval respiration decreased until December, then increased slowly. Larval respiration increased before pupate in March。...