| RAPD analysis was used to evaluate genetic relationship for 16 accessions germplasm resources of Astragalus membranaceus,collected from abroad and different areas in our country.And effective component analysis was carried out to screen the highest germplsm of effective component for them with UV spectrophotometric method.The aim was to establish the foundation for A.membranaceus breeding and genetic improvement.The main results were summarized as follows.1.Optimization of RAPD Reaction System for A membranaceus was founded.That was in a 20-ml volume,5 ng template DNA,0.4μmol/L primer,150μmol/L dNTP,2.0 mmol/L MgCl2,1 unit of Taq DNA polymerase and 1×Buffer.Amplification protocol was initial denaturation of 5 min at 95℃;40 cycles of 45 s at 95℃,60 s at 36℃,and 90 s at 72℃and a final cycle of 7 min at 72℃.2.16 accessions of A.membranaceus germplasms was analyzed by using RAPD markers. 12 polymorphic primers,screened out from 200 random primers,were used and produced 148 reproducible bands of them,127 bands(85.8%) were polymorphic.The cluster analysis divided the germplasm used in this study into 3 groups:wild A.membranaceus,north China A.membranaceus and northeast A.membranaceus.3.There was significant difference(1%) among different germplasm resources. Polysaccharides,saponins and flavonoids of wild A.membranaceus from Jilin-Helong were significantly higher than cultivated A.membranaceus.In cultivated A.membranaceus, polysaccharides of Jilin-Tonghua was the highest but saponins and flavonoids were lower; saponins and flavonoids of Jilin-Baishan were lower but polysaccharides was the highest. Therefore,in cultivation,we should select different germplasm with different goal. |
