The Relation Between Ascorbic Acid Contents And Activities Of Related Enzymes In Vegetable Species

The leaves of spinach , potato and sweet pepper and the product organs of sweet pepper, tomato, potato, radish and cucumber that AsA contents have significant difference were used to investigate the relation between AsA contents and relative enzymes activities at different development stages.The results showed as fllows:1. AsA contents in product organs of sweet pepper, tomato, potato, radish and cucumber increased during the development,reaching maximum at anaphase of product organs.The results showed that changes of AsA contents were accordant with GalLDH and DHAR activities and AsA+DHA contens were accordant with GalLDH activities in product organs at different development stages.2. Correlation between AsA contents and relative enzymes activities at anaphase of product organs showed that AsA contents and DHAR activities were significantly positively correlated(r=0.9423, P<0.05), AsA contents and GalLDH activities were considerably positively correlated ( r=0.8597, P<0.10),AsA+DHA contents had clear pertinence with GalLDH activities(r=0.7904, P<0.15),but AsA contents had no clear pertinence with MDHAR,AAO and APX activities.3. The result from the experiment indicated that AsA contents were strongly affected by the GalLDH and DHAR activities, but MDHAR,AAO and APX activities were not the main factors in influencing the AsA contents in product organs of different vegetable species.4.In the leaves of spinach, potato and sweet pepper, AsA and AsA+DHA contents reached minimum in aged leaves, GalLDH activities reached minimum in aged leaves too.The changes of GalLDH activities were accordant with AsA and AsA+DHA contents. It explained that GalLDH activities can be one of the indirect sign of the AsA contents in the leaves of spinach, potato and sweet pepper.5.DHAR,MDHAR and GR activities reached minimum in aged leaves,and the changes of DHAR activities were accordant with AsA and AsA+DHA contents in the leaves of spinach and potato. AsA contents had no clear pertinence with AAO and APX activities, it is proposed that AAO and APX activities were not the main factors in influencing the AsA contents in vegetable leaves.