Development Of Pol CMS Restorers With Resistance To Sclerotinia Sclerotiorum In Brassica Napus

China is the largest nation for Brassica napus production in the world. The area of the planting has exceeded 7,337,000 hm² and both the area and the total amount are one third of the entire production of the world. The drainage area of the Yangtze River provides the largest yield area of Brassica napus all over the world, which almost occupies one fourth of the whole earth. Brassica napus contains both high oil and high protein and it is widely cultivated in Canada, West Europe, North Europe, Australia, India and so on. Brassica napus is also one of the main resource of edible oil and plant protein in the world.Sclerotinia sclerotiorum, which is one kind of serious diseases that confines development of Brassica napus and causes significant production reduction. Particularly, it is during the period of high temperature and rainy days that the Brassica napus come on disease more seriously on its later growth phase in center down main yield areas of Yangtze River. Thus, it is exigent and desirable to select disease-resistant new varieties and germplasm resources.This study focus on selection of disease-resistant restorer lines through two approaches of microspore culture and marker assisted selection. On the one hand, we derives disease-resistant novel restorers on the basis of disease-resistant restorer ZHONG SHUANG 4 RDH and non-resistant restorer 5148 DHL sterility 109A, using microspore culture of Brassica napus to obtain doubled haploid (DH) lines of separation population between resistant and sensitive disease plants and through identification of disease-resistant in the field. On the other hand, in order to derive novel disease-resistant restorer lines, we use marker assisted selection to improve resistance property of smart materials and select materials both having genetic background consistent with recurrent parent and display disease-resistance in identification of disease-resistant experiment. The results are as follows:1. Through microspore culture for F₁ generation of (5148 DH×ZHONGSHUANG 4 RDH) and (109A×ZHONGSHUANG 4 RDH), we derived 438 and 171 different genotype DH single plants respectively.2. When identification of disease-resistant for 2 DH populations in the seedling stage, we obtained 69 and 39 different genotype disease-resistant single plants respectively.3. When identification of disease-resistant for 2 DH populations in the adult plant stage, we derived respectively 38 and 17 different genotype disease-resistant single plants; Simultaneously there were quite correlation on length of disease spot between 3d and5d, 5d and 7d,3d and 7d. but we could not find any single plant of immunity and resistant enlargement for Sclerotinia sclerotiorum.4. There were 9 single plants, which showed resistance property both on seedling stage and adult plant stage. And there was no significant correlation on disease-resistance between seedling stage and adult plant stage.5. There were 4 single plants, which both had the same genetic background with 5148 DH using marker assisted selection and through backcross twice and selfing once and displayed disease-resistance. These single plants might be as novel restorer lines germplasm resources.