Allelopathy Of Hemarthria Compressa And The Identification Of Chemical Components

Hemarthria compressa is a superordinary pasture,but its quality and yield will degrade with ripe.The problem will be solved by mixtusing with legume.This paper examined the feasibility by studying allelopathy of H.compressa on legume(T.repens and T.pratense).And the allelopathic effect of extraction solution of shoot and leaf,volatile oils of shoot and leaf and root exusates on two legumes were studied using the bioassay method in lab.Subsequently,potential allelochemicals of extraction solution of shoot and leaf,volatile oils of shoot and leaf and root exusates were analyzed by GC-MS.The main result showed as follows.1.The allelopathic effects of extraction solution of shoot and leaf,volatile oils of shoot and leaf and root exusates of H.compressa on two receptor plants were bioassayed in lab,the results indicated that germination and seeding growth were significantly inhibited, the inhibiting effects to receptor seedling were stronger than germination.The influence to the seed had two forms,inhibiting and delaying germination.The length of shoots and roots was inhibited and the seedling had malformed symptorn.The allelopathic effects of extraction solution of shoot and leaf＞the allelopathic effects of volatile oils of shoot and leaf＞the allelopathic effects of root exusates.2.The allelopathic effects of H.compressa on T.repens and T.pratense were studied, the result indicated that the effect of H.compressa on the inhibiting and delaying germination of T.repens was stronger than the effect of T.pratense in the same concentration.And the effect of the extracts of H.compressa on the seedling growth of T. repens was stronger.So T.pratense was chosen as the best companion species for H. compressa.3.The extraction solutions,volatile oils,root exusates,were analyzed by GC-MS.The result indicated that eighteen compounds were detected in extraction solution of shoot.And mainly include aldehyde,acid,diolefin,Phenols,amine,and so on.The main compinents of extraction solution of shoot included Phenol,2,4-bis(1,1-dimethylethyl)-,Hexadecanoic acid and Linoleie acid.It was reported that they were allelochemicals.In extraction solution of leaf was detected there were twenty-six compounds.They were diolefine,diolefine,ester and acids.Hexadecanoic acid and Tetradecanoic acid were higher relative amount.In the detected compounds,Linoleic acid,9,12,15-Octadecatrienoic acid(Z,Z,Z)-and Hexadecanoic acid were reported that they were allelochemicals.There were eleven same compounds in shoot and leaf.They were tetradecane,tetradecane,pentadecane,2,4-bis(1,1-dimethylethyl)-,hexadecanoic acid,daturic acid,tetradecanoic acid,hexadecanoic acid,linoleic acid,tricosane and lignocerane.4.The volatile oils were analyzed by GC-MS.The result indicated that there were twelve compounds in volatile oil of shoot.They were alkenes,diolefine and acids. Phenol,2,4-bis(1,1-dimethylethyl)-,Hexadecanoic acid,N-hexadecanoic acid and Linoleic acid were higher relative amount in volatile oils of shoot,and first three of the four compounds were reported that they were allelochemicals.There were twenty compounds in volatile oil of leaf.And 3-hexen-1-ol,Phenol,2,4-bis(1,1-dimethylethyl)- and Tetradecanoic acid were higher relative amount.In the detected compounds, benzaidehyde,phytol and 9,12,15-Octadecatrienoic acid(Z,Z,Z)- were reported that they were allelochemicals.5.The root exudation was analyzed by GC-MS.The result indicated that there were twenty compounds in root exudation.They mainly include aldehyde,alcoh,diolefin,amine,alcohol phenyl and acids.In root exusates Benzaldehyde,Benzyl Alcohol and Hexadecanoic acid were higher relative amount.They were reported that they were allelochemicals.