| China is a major area for origin and genetic variation of walnut, which has abundant nutrimental material, vitamin, and it has a long planting history. It is an important fruit and woody species. The genetic diversities of 35 walnuts samples derived from 7 different regions in Plateau of Western Sichuan Province and Qinba mountainous regions were investigated using AFLP and RAPD methods. To combine with morphological and agronomical traits, the results were as follows:1,Based on the genomic DNA extracted from Walnut, essential factors affecting the result of RAPD and AFLP assay were tested and compared. The research approved that an optimal reaction system was suitable for the assay and usage of RAPD and AFLP in Walnut.2,In the research, 324 RAPD fragments were produced, 279(86.1%) of total bands showed polymorphic with the size ranging from 180 to 2000bp, and 10.1 fragments per primer were amplified on average; 769 AFLP fragments were showed by twenty eight primers, and 440 polymorphic bands were generated, the percentage of polymorphic bands was 57.2%.3,Walnut in Plateau of Western Sichuan Province showed highest polymorphism detected by both RAPD and AFLP markers. The high number of alleles and the high expected genetic diversity detected with RAPD and AFLP markers make them a suitable tool for walnut cultivar identification, confirming these markers transportability among general in the walnut family.4,In the study, RAPD markers detect a few genetic loci than AFLP per primer. Although the level of polymorphism offered by AFLP was lower than RAPD, AFLP was efficient to reveal more polymorphic bands in a single lane than RAPD. Additionally, false positive products were easily recognized in AFLP, and sequence gel with high resolution and adaptor techniques used in AFLP analyzing that allows a more precise identification of similar and dissimilar AFLP allele. The study indicated that AFLP marker were much more reliable and provided a more coverage throughout the genome than RAPD. |
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