Efficiency Of Symbiosis Between Lanzhou Lily (Lilium Davidii Var. Willmottiae) And Arbuscular Mycorrhizal Fungi

Lanzhou lily (Lilium davidii var. willmottiae) is the only sweet lily in China. It is well-known for its rich nutrition and medicinal functions. Lanzhou lily grows on the hillslopes and terrains at the altitudes 1900-2300 meters, where the climate is semi-arid. Arbuscular mycorrhizal (AM) association is the most prevalent mutualistic association between plants and microbial organisms, which is estimated that more than 80% of all terrestrial plants form this type of association. Arbuscular mycorrhizae play a key role in natural ecosystems and influence plant productivity, plant nutrition acquisition and plant community structure. In order to discover the effects of AM on the growth of Lanzhou lily, field experiments were carried out to study the effects of AM fungi on plant growth and bulb production in Lanzhou lily.1. Results showed that AM fungi successfully colonied the roots of Lanzhou lily forming Arum-type mycorrhiza. The different contents of phosphorus significantly influenced the growth and development of AM fungi (P<0.05). The mycorrhizal colonization rates significantly declined with increasing phosphate levels (P<0.05). In the low level of phosphorus treatments, benonyl significantly inhibited the growth and development of AM fungi (P<0.05).2. AM colonization rates extremely significantly increaseed with the growth of Lanzhou lily (P<0.01), showed that the different developmental stages of Lanzhou lily influenced the growth and development of AM fungi. AM colonization rates in seedling stage were the lowest and in bulb growing stage were the highest. Then AM colonization rates began to decline in dormant stage.3. Total P contents and total N contents of bulbs were higher than those in roots of all the treatments (P<0.05). Total P contents of bulbs increased with increasing phosphate levels. Results showed that increasing the phosphorus levels could not promote total N contents of bulbs and roots. In the low level of phosphorus treatments, benonyl influence the total P contents of bulbs and roots significantly (P<0.05).4. The biomass of bulbs of Lanzhou lily increased with the increasing phosphate levels. In the low level of phosphorus treatment, the biomass of bulbs significantly decreased after using benonyl (P<0.05). In the high level of phosphorus, the influence of benonyl was not significantly (P>0.05).5. Variety of acid phosphalase (ACP) and alkaline phosphalase (ALP) activity under different phosphorus levels in the rhizosphere and non-rhizosphere of Lanzhou lily was studied. Result indicated that, when the phosphorus contents in the soil increased, ACP and ALP activity in the rhizosphere soil of Lanzhou lily decreased (P<0.05). ACP activity was higher than ALP activity in the rhizosphere soil of Lanzhou lily (P<0.05), while ACP activity was lower than ALP activity in the non-rhizosphere soil of Lanzhou lily(P<0.05).