Establishment Of Method Of Culture And Detection Of C.JEJUNI And Investigation Of Situation About Infected Livestock And Avian In Shanghai

Campylobacter jejuni widely distributed in nature, is a food-derived pathogens. The bacteria infection people, it can cause human fever, acute colitis, food poisoning and Guillain-Barri syndrome; can cause cattle and sheep abortion, turkeys hepatitis and blue crown disease, and chicken necrotizing hepatitis, chicken, calf, piglets diarrhea and other diseases on human health and livestock production have caused great harm.In this experiment, ordinary candle-cylinder based on the law , pyrogallic acid and anhydrous sodium carbonate in the amount of 2.5 g / L, while a combination of improved Camp-BAP medium has greatly enhanced the Campylobacter jejuni Isolation efficiency. On this basis, the establishment of a rapid, concise and accurate PCR detection method, The detection method-specific, and highly sensitive detection (6 cfu / ml), rapid detection, 24 h given test results.Application has been established by the PCR method to detect from 2005 to 2007 in three poultry markets in Shanghai and five live pigs slaughter house of 2234 (chicken 1015 and swine 1219)cecal content of samples PCR analysis. The results showed that chickens infected with the average positive rate was 28.4 percent, pig infected with the average positive rate was 34.4 percent.Analyzed by SAS, samples of swine was significant higher in C.jejuni infection positive rate than that of chicken. Swine and chicken from Shanghai showed significant lower C.jejuni infection positive rate than that from Zhejiang Jinhua, Jiangsu Haimen, Jiangsu Zhangjiagang Jiangsu Dafeng and other habitat.