Identification Of Flies Species Around Camel's Living Environment And Analysis Of Internal Transcribed Spacers Of Horn Flies

To ascertain the vector of Parabronema skrjabini Disease and found a fast and accurate molecular biological technique for screening vector, flies were taken from bactrian camels around their living environment and were identified by traditional morphological method. Internal transcribed spacer of P. skrjabini was amplified from all flies species by PCR with primers that designed according to the sequence of P.skrjabini. And the sequences of ITS of Haematobia irritans and Haematobia titillans, the doubtful vectors, were sequenced and were analyzed with DNAStar5.0 software. The results showed that there were 3 families, 5 genera, 11 species'flies around camel's living environment; one fragment, whose sequence homology with ITS of P. skrjabini was 99.2%, was amplified from H. irritans. The length of ITS of H. irritans and H. titillans were 994bp and 962bp respectively, the similarity and variability of ITS between H. irritans and H. titillans were 86.8% and 14.9%, and there were 190 recognition sites. This paper described morphological character of all flies species around camel's living environment detailedly, and also an identification key of them was made and a great deal of color pictures of flies was attached. This research explored a more timesaving and laborsaving molecular biological method than traditional vector screening method, filled the blank in molecular biological researches of H. irritans and H.titillans to some extent, and supplied molecular recognition mark for the identification the larvae of H. irritans and H. titillans. All of the achievements found an important basis for studying the vector of P. skrjabini.