Study On MRNA Differential Expression Between Male Sterile And Fertile Flower Buds Of Flax And Analysis Of Differential Fragments

The mRNA differential expression was compared by mRNA differential display PCR (DDRT-PCR) between male sterile and fertile flower buds of flax. The positive differential fragments were confirmed by Reverse Northern Dot Blotting, and then sequence analysis of these fragments was carried out with the main results as follows:(1) The optimum conditions of DDRT-PCR system of flax were established.(2) Among the 52 fragments that had been obtained, 36 fragments were from male sterile flower buds; the other 16 fragments were from male fertile flower buds.(3) 9 fragments were confirmed by Reverse Northern Dot Blotting. 5 fragments were specific to male sterile flower buds: G+E07+100bp, G+E07+330bp, G+E07+830bp, G+S273 +500bp, A+S267+300bp; 2 other fragments were specific to male fertile flower buds: G+S274-250bp, G+S274-450bp.(4) The positive fragments were sequenced and analyzed with BLAST. The G+E07+100bp which is homologous to GTP-binding protein of plant suggested that it may play an important role in signal transduction. The G+E07+330bp may be closely related to fertility. The G+E07+830bp, which is most similar to the sequence of Beta-D-xylosidase, may influence carbon hydrate metabolism in flax anther and pollen, resulting in pollen abortion. The G+S273+500bp is homologous to NAD+ADP ribosyltransferase. The A+S267+300bp, homologous to secreted protein, may play roles in signal transduction, Morphogenesis and Apoptosis in flax and finally lead to male sterility. The G+S274-250bp and G+S274-450bp had no significant similarity with any known genes in plants, so they may be newly found genes related to fertility in flax.