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Isolation And Characterization Of A Aeromonas Caviae Bacteriophage

Posted on:2009-12-27Degree:MasterType:Thesis
Country:ChinaCandidate:J X DengFull Text:PDF
GTID:2143360245957865Subject:Microbiology
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A bacteriophage, was isolated from bacterium Aeromonas caviae from East Lake, Wuhan city, China. Morphologically, the phage has a typical Myoviridae structure consisting of an isometric head (50 nm in diameter) and a visible tail. The bacteriophage has a latent period of about 90 minutes and an average burst size of about 125 PFU·Cell-1. Restriction enzyme pattern of the bacteriophage's genome showed that the genome is a double-stranded DNA and about 23kb in size. The sequenced genomic fragments showed highly similarities to gp04 and gp16 sequence of other Myoviridae bacteriophages at protein level.combined with the information about electron microsopy and nuclease figures, the phage was considered Myoviridae. The phage-host system make sense to the research of fish disease and freshwater ecology.
Keywords/Search Tags:phage-host system, Aeromonas caviae, isolation and identification
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