Identification And Selection Of Stripe Rust Resistance Lines From Derivatives Of Crossing Between Triticum Monococcum And Avena Fatua And The Establishment Of GISH Technology

Wheat stripe rust was caused by Puccinia and it was the primary world-wide low-temperature disease and one key factor limiting the yield of wheat in China was the largest occurrence land of wheat stripe rust in the world, particularly in Huang-Huai and Yangtzi river valley, stripe rust has been the main wheat disease. Because of the rapid variation of the stripe rust races,some resistant genes in wheat have lost their resistance. So there is a great need to find and introduce new resistant genes to wheat varieties and enhance their disease resistance. It is known that the relative species of common wheat are the good resources of resistance genes. Avena fatua L is one wild relative of common wheat with many excellent properties, such as resistance to many diseasesearly-maturation and has,drought resisting. It contains high fine character in protein and the lysine content .It contains good idioplasm resources for the wheat breeding. Professor Zhang Qingqin used Triticum monococcum and Avena fatua for hybridization and obtained hybridization descent series named yilipu.Morphological Stat, Identification and selection of stripe rust resistance , cytological, in situ hybridization, were employed to detect and analyze from Derivatives of crossing between Triticum monococcum and Avena fatua. Major result is as follows:1. Field investigation indicated that derivatives of crossing between Triticum monococcum and Avena fatua line had good phenotype and looked excellent.This result indicated that the progeny have potential use in wheat breeding.2. This study tested with a set of Chinese Pst races in crossing descendants of Triticum monococcum and wild oat and those homozygous for resistance to stripe rust were tested with a set of 9 stripe rust races. The oat proved to carry a number of genes for resistance to stripe rust. Eight selected lines, YLP-7, YLP-1-1, YLP-1-6, YLP-9-3, YLP-9-8, YLP-15-1, YLP-15-4 and YLP-16-4, were resistant to all tested races of Chinese Pst. This result indicated that the progeny have potential use in wheat breeding.3. The mitosis metaphases of the root cells of 8 resistance lines were observed. It was showed that the chromosome number in all samples is even, giving 2n=42, 44, 46,56.It was showed it was maybe derivatives of crossing between Triticum monococcum and Avena fatua. 4. Factors influencing genomic in situ hybridization (GISH) used in Filial Generations from Distant hybridization of Avena fatua and Triticum monococcum .The results indicated that ideal GISH technique were based on the high quality chromosome preparations containing more cell mitotic metaphase.The critical factors were suitable ratio of probe DNA to blocker DNA.In addition, some phenomenas were analyzed. Proposals and solutions for these problems were further brought forth..