Study On The Genetic Correlation Between KAP Gene With Partial Economic Traits In Tibetan Sheep

254 individuls of Tibetan sheep which classified three ecological types named plateau, Oula, Qiaoke, were selected for analyzing the polymorphism of partial sequence of KAP3.2, KAP6.1, KAP7 and the exon of KAP8 by PCR-SSCP and gene sequencing. In order to analyze the genetic correlation between the polymorphism and the traits of weight, wool length, wool yield, the least square linear model was used to determine the preponderant gene locus, the selection reaction were calculated via genetic correlation between partial economic traits and marker loci, providing theoretical basis for MAS in Tibetan sheep. The results as below:1. The analysis results showed that there are polymorphism at the loci of KAP3.2,KAP6.1,KAP7 and KAP8 in all three ecological types.of Tibetan sheep. Three genotypes of AA, AB and BB were detected at four loci in all three types, but only two genotypes of AB and BB at KAP8 in Qiaoke. The sequencing indicated there was a mutation of C→T at 271bp of KAP3.2, and a mutation of T→C at 1122bp of KAP8, they both synonymy mutation, and a mutation of T→C at 102bp of 5′UTR of KAP7.2. The population genetics analysis showed: In Plateau type, Gene A was preponderant allele at KAP3.2, and B in Oula and Qiaoke. At KAP6.1,KAP7 and KAP8, the distribution of alleles are highly consistent, the frequency of Gene B was much higher than A. KAP8 in Plateau,KAP7 in Oula and Qiaoke,and KAP3.2 and KAP6 in all three types of Tibetan-sheep were in medium polymorphic. KAP8 in Plateau and Oula,KAP7 in Qiaoke,and KAP3.2 in all three types of Tibetan-sheep were in Non-equilibrium of Hardy-Weinberg's.3. Effect analysis of genotype showed: (1) The least squares mean (LSM) of Genotype BB of KAP7 was significantly higher than AA and BB(P﹤0.01) in Plateau and Qiaoke, BB of KAP6.1 and AA of KAP8 were significantly higher than AA(P﹤0.05)and BB(P﹤0.05) in Oula respectively, the selection reaction of KAP6.1(0.9153) was higher than KAP8(0.8905). So KAP7 can be used as a marker of body weight in Plateau and Qiaoke, KAP6.1 can be the preponderant marker locus for body weight in Oula. (2)To the trait of wool length, the LSM of genotype AA of KAP3.2 and AA of KAP6.1 were higher than BB(P﹤0.05)and AB(P﹤0.05)in Plateau respectively. In Oula type, AA of KAP3.2 was significant higher than BB(P﹤0.01), and AA of KAP6.1 higher than AB(P﹤0.01), AA of KAP7 was higher than AB, BB(P﹤0.05). In Qiaoke, AA of KAP3.2 was significantly higher than AB(P﹤0.01), AA of KAP6.1 was significantly higher than AB and BB(P﹤0.05), AB of KAP8 was significantly higher than BB(P﹤0.01).The selection reaction of KAP3.2 in Plateau and Oula type are both higher than the others, can be used as a preponderant marker, so does KAP8 in Qiaoke. (3)To the trait of wool yield, the LSM of genotype AA of KAP3.2 was higher than AB(P﹤0.05), and AB of KAP8 was higher than AA and BB(P﹤0.05). In Oula type, AA of KAP3.2 was higher than AB and BB(P﹤0.05), and BB of KAP6.1 was significantly higher than AA and AB(P﹤0.01), AB of KAP8 was higher than BB(P﹤0.05). In Qiaoke, AA of KAP3.2 and KAP7 were significantly higher than AB and BB(P﹤0.05), AB of KAP8 was higher than BB(P﹤0.05). The selection reaction of KAP8 in Plateau and Oula type are both higher than the others, can be used as a preponderant marker, so does KAP7 in Qiaoke.