Inspection Of Garlic Virus Disease And The Identification And Sequence Analysis Of Onion Yellow Dwarf Virus In Garlic From North Xinjiang

The garlic is one of important vegetable crops Xinjiang. The virus disease with stripe mosaic,stunt,distorting leaf , necrosis effect on the production and quality of garlic in North XinJiang. In order to make clear the sorts of virus in garlic virus disease , in 2007,garlic virus diseases main regions were investigated,in Urumchi,Shihezi,Jimusaer,Yilizhaosu and 76 state farm and so on. By electron microscope observation ,the virus particles that is 780×11nm were observed.Some special primers were designed based on generally reported virus gene sequences in garlic ,only a fragment of about 1402bp was amplified by RT-PCR using the total RNA of the garlic infected by OYDV special primer. The products of PCR were cloned into PMD18-T and transformed into TOP10. Through clony PCR detection, the fragments obtained are about 1402bp. The five combined clones were sequenced, and analyzed by DANMAN soft , the result showed that the fragment is 1402bp, encode 467 amino acids . The five clones form two groups, the obvious difference were seen between the two groups. In one group , including XJJM-3 and XJJM-6, had the highest nucleic acid sequence identity 99% and amino acid sequence identity 98% with AJ409311 from Shandong province Jinxiang of China. The other group consisted of XJJM-2, XJJM-4 and XJJM-5, the homology could reach 99%of nucleic acid and 99% of amino acid, compared with AB000481 from Japan, the result showed that the homology could reach 98%of nucleic acid and 98%of amino acid.