Establishment And Application Of Methods For Drug Residues Of Different Class In Animal-Borne Food

The most serious objections to the presence of chemical residues in food intended for human consumption arise as a consequence of human health considerations. A wide range of physicochemically and structurally highly different compounds may be used illegally or entered unexpectedly the food chain via contamination of animal feeds and food production. Since food safety has become one of the most vexing problems and the main objectives of the regulatory control of chemical residue are to ensure a safe and wholesome food supply and to take regulatory action after identification of adulterated products, analysts are usually challenged to analyze.This paper discusses the regulatory by different organizations and nations, combines the current official analytical methods of China, and seeks to provide ideas for better approaches to existing analytical methods. We established 4 methods for drug residues of different class in animal-borne food. We tested the efficiency of the methods by FAPAS Proficiency Test and applied this method on the routine work. The paper contains 5 chapters covering topics related to chemical residues in food.Chapter 1 discusses issue of the food safety in China and the comparison of the regulatory outlines all over the world, and seeks to figure out the ways to improve the food safety.Chapter 2 discusses the analytical method ofβ-agonists in Foodstuffs of Animal. We established LC/MS/MS method to detect clenbuterol, terbutaline, salbutamol, zipaterol, bromotuterol, cimaterol, clenbupenterol, mabuterol, tulobuterol and mapenterol. Recovery ofβ-agonists in fortified samples varied from 60.3%to 115.3%.LOD varied from 0.04 to 0.33μg/kg. In PAPAS Proficiency test, Z- score for clenbuterol is 0.2 and for ractopaime is 0.6.Chapter 3 discusses the analytical method of Sulfanilamides in animal-borne food. Weestablished LC/MS/MS method to detect Sulfanilamides in milk and meat. Recovery of 16 Sulfanilamides in fortified meat varied from 62.2%to 98.0%. LOD varied from 0.08 to 1.03μg/kg. Recovery of 9 Sulfanilamides in fortified milk varied from 92.9% to 106.7%. LOD varied from 0.08 to 0.69μg/kg. In PAPAS Proficiency test, Z- score for sulfamethazine is -0.3, for sulfamethizole is -0.2, for sulfapydidine is -0.1, and for sulfisoxazole is 0.2. Chapter 4 discusses the analytical method of tetracyclines in animal-borne food. We established LC/MS/MS method to detect tetracyclines in honey, egg and meat. Recovery of oxytetracycline, tetracycline, chlortetracycline and doxycyclin in fortified honey varied from 59.7% to 82.9%.LOD varied from 0.0004 to 0.0010mg/kg. Recovery of oxytetracycline, tetracycline, and chlortetracycline in fortified egg varied from 66.8% to 89.5%. LOD of each was 0.005mg/kg. Recovery of oxytetracycline, tetracycline, and chlortetracycline in fortified meat varied from 77.0% to 87.7% .LOD each was 0.005mg/kg.Chapter 5 discusses the analytical method of quinoloines in animal-borne food. We established LC/MS/MS method to detect 11 quinoloines in egg and meat. Recovery of 11 quinoloines varied from 46.1 % to 109.2%.LOD varied from 0.013 to 0.116μg/kg.