Localization And Express Of RCC1 In Oocyte Maturation Fertilization And Early Embryonic Development Of Mouse

RCC1 is the only known guanine nucleotide exchange factor for the small GTPase Ran and acts as a Cell Cycle Regulator throughout the cell cycle in mitotic. Inside the cell, RCC1 is essential for the efficient conversion of RanGDP to RanGTP. It is essential for several key cellular processes, including nucleocytoplasmic transport, regulation of spindle formation and nuclear envelope reassembly at mitosis, and prevention of rereplication of DNA during S phase.In this experiment, first we used the nested PCR to examine RCC1 of the mouse oocyte, and early cleavage, with the development of the early cleavage, the express of RCC1 was up-regulated. Then we investigated the possible localization of RCC1 during mouse oocyte meiotic maturation, fertilization, and early cleavage by using confocal laser scanning microscopy. The result shows that RCC1 was localized mainly on the nucleus (except for the nucleolus) microamount disposition in cytoplasm in oocyte, zygote and early embryos,after the nuclear membrane breakdown,RCC1 distributed throughout the cell, but is not observed predominantly concentrated around the condensed chromosmomes.After sperm penetration, RCC1 is dispersed with the extrusion of the second polar body and gradually concentrated in the male and female pronuclei .The possible localization of RCC1 in mouse testis sertoli cell. RCC1 is distributed mainly on the nucleus except the nucleolus in interphase stage, In mitosis stage, after the nuclear membrane breakdown, RCC1 distributed throughout the cell, The localization of RCC1 in mouse testis sertoli cell was just like that in mitosis of early embryo cell.Our results indicate that RCC1 plays an important role in the regulation of cell cycle progression, nucleocytoplasmic transport ,nuclear breakdown/reassembly and microtubule organization in mouse oocytes, fertilized eggs and early embryos, the result just as found in other organisms in interphase stage in vivo and in vitro, but there is no special distribution around the chromosome in cell division phase.