Development Of A PCR-RFLP Assay For Genotyping Porcine Circovirus Type (PCV) And Genetic Variations Of PCV Isolates Prevalent In China

Porcine Circovirus type 2 (PCV2) infection is an important infectious disease in pigs in recent years, which seriously hinders the development of pig industry. PCV2 infection can cause many diseases, such as Postweaning Multisystemic Wasting Syndrome(PMWS), Porcine Reproductive Disease Syndromes(PRDS), Porcine Dermatitis and Nephropathy Syndrome(PDNS), Congenital Tremors(CT), Interstitial Pneumonia(IP), Porcine Proliferative and Necrotic Pneumonia(PNP), and so on. PCV2 infection can depress the body immunity, cause secondary infection by other pathogenic microorganisms and aggravate the pathogenetic condition. The disease spread widely in many countries and areas and bring big economic loss in pig industry all over the world. There are a lot of reports about the prevalence of PCV2 in China in succession. Zhang jia yong and so on, has already carried on the PCV2 serosurvey in ten pig farms of six places in Heilongjiang Province. And many borderline cases in clinic have been found in hei long jiang province.The report of Investigating serology has existed, whereas no having the proof of pathogeny diagnoses. In order to offer a credible mean to detect the pathogeny in lab,the method of PCR for PCV has been established based on the rerults of our lab that can detect PCV fast. Based on the full gene sequence of PCV2(AF027217), a pair of specific primer was designed and synthesized which includ the specificity enzyme to cut the position with EcoR I; Based on the full gene sequence of PCV1(AX092145), a pair of specific primer was designed and synthesized which includ the specificity enzyme to cut the position with Hinc II. Meanwhile we analyse the PCV2 ORF2 isolated from Dong three province on genetic variation. The PCR diagnosis method of fast, convenient, keen of detecting PCV is successfully established, is suitable in the location examines PCV.2. Nine PCV2 were isolated from sample of location isolation. the homology is higher than 97%, The six PCV2 is in the identical branch on the cladogram with domestic separation. The investigation result indicated that the northeast three provinces pig group has the PCV2 infection generally. PCV2 may infect the different age; The infection of new-born pig is high, but the infection percentage to the different pig farm exists difference, this main related with the raising management condition and the immunity condition of the different pig farm; The fat infected PCV2 often present inapparent infection, its infection percentage exists obvious area difference. Three PCV1 were sucessfully isolated from sample of location isolation.Sucessfully clone the one PCV1 genes: J5-PCV1 1758bp;G1-PCV1 1759bp;Wen-PCV1 1759bp;...