| Mutants were one of the most important materials for function genome research.Many of them had been used for gene mapping and gene cloning. Three rice mutants,which were spot leaf mutant M14,rolled leaf mutants B157 and B369,were identified by classical genetical analysis in this paper.Also,we had primarily mapped url1(t)(unilateral rolled leaf 1)to chromosome 1.The main results were here:1,Six F2 populations were developed from three mutants.In order to confirming whether the three mutants were controlled by recessive gene or dominant gene and controlled by a single gene or not,we investigated the segregation ratio of character in every population.It was suggest the mutant phenotypes of M14 and B157 were controlled by a single recessive gene,while B369 was controlled by a semi-dominant gene.2,A mapping population including 58 mutant phenotype plants was derived from crossing between Super Rice in Toxyox(ssp.indica)and B157.With this mapping population,a rolled leaf gene url1(t)from B157 was primarily mapped to the site of 30cM on Chromosome 1.So far,It was not report there was a rolled leaf gene at this site,so we concluded that url1(t)was a new rolled leaf gene in rice.Two molecular markers RI02526 and RM272 closely linked to url1(t)were obtained on two sides,the genetic distance between them and url1(t) was 2.0cM and 0.6cM respectively.RI02526 and RM272 were located on BAC clones AP003200 and AP003052,a 692.9kb region containing url1(t)gene was defined with the two markers,including 78 putative genes.3,Red all putative genes in the 692.9kb region in TIGR database annotating rice genome.We didn't find miRNA gene and the homologous gene coding HD-ZIPâ…¢and AGO proteins related to leaf-adaxial development,but we found five putative genes which might be associated with the rolled leaf gene url1(t). The five putative genes were gene:11971.t00888,gene:11971.t00889, gene:11971.t00886,gene:11971.t00917,gene:11971.t00935.gene:11971.t00888 and gene:11971.t00889 were located on BAC clones AP003200,coded RNA-dependent RNA polymerase family protein;Gene:11971.t00886 was located on BAC clones AP003200,coded cytokinin dehydrogenase 1 precursor; Gene:11971.t00917 was located on BAC clones AP003197,coded cell division related protein;Gene:11971.t00935 was located on BAC clones AP003105, coded BES1/BZR1 homolog protein 4,BES1/BZR1 was the key transcription factor in regulation of auxin. |
