| 1-MCP (1-Methylcyclopropene) could competitively combine with the ethylene receptor, block ethylene biosynthesis and its physiology effects, thus it could retard the ripening and senescence of fruit. The effects of 1-MCP were proved by many fruits and vegetables experiments, and were used in practical application. According incomplete statistics, 60,000 tons of apple fruits were stored by using 1-MCP in Shan Dong province in 2004, the number was 100,000 tons in 2005.Many diseases happened in horticultural products'storage. This must be considered before realizing the gross business application of 1-MCP. In recent years, the effects of 1-MCP on physiological diseases were reported more than on infectious diseases. The purpose of this experiment was on the latter aspect, and we hope that it could provide support for the further practical application of 1-MCP.'Fuji'apple fruits were the experimental material. The fruits were inoculated with Botrytis cinerea and Gloeosporium fructigenum Berk separately after fumigated by 1-MCP, and then they were stored in normal temperature. The main results were as follows:(1) The fruits of'Fuji'apple treated with 1-MCP had lower respiration and ethylene production than the ones untreated. Different fungi had different responses on 1-MCP. Among the fruits inoculated with Botrytis cinerea, all the treatments of 0.25,0.5,0.75μl·L-11-MCP had smaller putrescence area than the control. Among the fruits inoculated with Gloeosporium fructigenum Berk, the 0.5μl·L-11-MCP treatment had smaller putrescence area than the control, the 1.0μl·L-11-MCP treatment had no obvious difference from the control. It also showed that the shaded side had bigger putrescence area than the side toward the sun.(2) The SOD,POD,CAT activities of the apples treated by 1-MCP were higher than control. But there was no obvious difference between the treatments. The treatments had less content of MDA than the control. It proved that the treatment of 1-MCP could delay destroy of plant cell membrane caused by fungi.(3) Among the fruits inoculated with Botrytis cinerea, the PAL activity of 0.5,0.75μl·L-11-MCP treatments were higher than the control. The PPO activities of all the treatments were lower than the control, and there was no obvious difference between the treatments. Among the fruits inoculated with Gloeosporium fructigenum Berk, the PAL activities of the two treatments of 1-MCP were higher than the control, and the 1.0μl·L-11-MCP treatment had higher PAL activity than the 0.5μl·L-11-MCP treatment. The PPO activities had the same manifestation as the fruits inoculated with Botrytis cinerea. Among the fruits inoculated with Botrytis cinerea, the 0.5,0.75μl·L-11-MCP treatments had lower content of total phenolic than the control. Among the fruits inoculated with Gloeosporium fructigenum Berk, the 0.5μl·L-11-MCP treatment had higher content of total phenolic than the control, the 1.0μl·L-11-MCP treatment had no obvious difference from the control. |
