Study On Staw Frozen Semen And Superovulation In Duolang Sheep

Duolang sheep originate from county of Maigaiti in south of Xinjiang, it has many peculiarities,such as mature early, high dressing percentage, high earnings of feedstuff, high quality of mutton, high rate of reproduction, appropriate for feeding in yard, and local people are fond of this type of sheep. Currently, breeding of Duolang sheep is mainly through natural mating.The efficient system of breeding hasn't been established. So variety preservation is imminent.To resolving these aspects of disadvantage, we try to carry out frozen-thawed semen and superovulation treatment in Duolang sheep, which have excellent genetic trait, to achieve presercation of Duolang sheep's variety.Experiment one: The objective of present study is to collect the semen of Duolang sheep , comparing the 5 frozen diluent ingredient ,preserve the semen by a straw , so as to do detecting of the mobility. The result indicates that 4.83% Lac diluent ingredient's frozed-thawed semen is average 36% ,which is better than the others, there is a significant different (P<0.05). Furthermore, 4.83% Lac diluent ingredient's abnormality rate was significant better than the others (P<0.05).It shows that 4.83% Lac diluent ingredient is the best of these ingredient on straw proceed ingredient.Experiment two: To study the optimal results of superovulation, 59 Duolang sheep were treated with 3 kinds of FSH products. The factors which influence the effects of superovulation in Duolang sheep were studied in this trial, such as the FSH product, methods of superovulation and dosage of hormone. The following results were obtained: (1)FOLLITROPIN-V was best one in 3 kinds of FSH products, the average number of transferable embryo was 7.27±5.26; (2)Effects of superovulation with natural mating was better than with CIDR inserted; (3)The purified domestic FSH product can obtained the preferable superovulation results.