Effects Of Vitamin E And Different Form Selenium On Milk And Plasma Fatty Acid Profiles And Activity Of Antioxidation In Dairy Cows Fed Fat Diets

Three experiments were conducted to investigate effects of vitamin E and differents form selenium dietary on milk and plasma fatty acids composition and antioxidation for dairy cow.ExperimentⅠ: Effects of Vitamin E and Selenized Yeast on Milk and Plasma Fatty Acid Profiles and Activity of Antioxidation in Dairy Cows Fed Fat DietsForty eight Chinese Holstein dairy cows were allocated into 4 groups as a 2×2 factorial randomized complete block arrangement for 12 cows per group. Cows were fed with basal diet with 2% soybean oil (control), or basal diet plus 13.6 g of selenized yeast/d (contain 13.6 mg of selenium/d) (SY), or basal diet plus 40 g of vitamin E/d (contain 20000 IU ofα-tocopheryl/d) (VE), or basal diet plus 13.6 g of selenized yeast /d (contain 13.6 mg of selenium/d) plus 40 g of vitamin E/d (contain 20000IU ofα-tocopheryl/d) (VESY). Dry matter intake, milk yield, and percentage and yield of milk fat, protein, lactose were not significantly different among treatments. Supplementation of vitamin E did not singnificantly effected the almostly all fatty acids of plasma and milk. While Supplementation of selenized yeast has singnificantly effected the almostly all of plasma and milk fatty acids. Supplementation of selenized yeast was significantly increased the percentage of DHA (Docosahexaenoic acid) (P<0.05), EPA (Eicosapentaenoic acid) in plasma fatty acids and the percentage of cis-9, trans-11 CLA (Conjugated linoleic acid) (P<0.001), PUFA (Polyunsaturated fatty acids) (P<0.05) in milk fatty acids. Meanwhile, the USFA (Unsaturated fatty acids) percentage of plasma fatty acids was decreased when supplementation of selenized yeast. TheΔ9-desaturase index did not significantly changed among treatment. Theα-tocopherol content of milk did significantly elevant follow selenized yeast addition (P<0.05). Theα-tocopherol content of plasma was increased also follow selenized yeast addition, but it was not statistically different. The selenium content of plasma and milk were significantly inreased follow the dietry selenized yeast addition (P<0.001). The activity of GSH-Px (Glutathione peroxi-dase) in plasma was increased when supplemention of vitamin E and selenized yeast, while, the activity of GSH-Px in milk did not chang, the activity of GSH-Px. in plasma and milk was not statistically different among treatments. The activity of SOD ( Superoxide dismutase) was tended to elevant follow the supplementation of vitamin E and selenized yeast (P=0.08). The activity of SOD and the content of MDA (Malondialdehyde) in plasma and the content of MDA in milk did not affect when vitamin E and selenized yeast addition. The quality of animal production had been elevanted when increasing the percentage of DHA, EPA in plasma fatty acids and the percentage of cis-9, trans-11 CLA, PUFA in milk fatty acids. Increasing special fatty acids with biological function in human diet was benefit to human health. The selenium andα-tocopherol concentration of milk and plasma in our study would partly satisfy the need of selenium andα-tocopherol for human by consuming dairy milk and meat. Increasing activity antioxidation of milk and plasma could benefet to protect the unsaturated fatty acids.The higher level vitamin E and selenized yeast of diet had not the affect infaust for dairy cows health.ExperimentⅡ: Effects of Vitamin E and sodium selenite on Milk and Plasma Fatty Acid Profiles and Activity of Antioxidation in Dairy Cows Fed Fat DietsForty eight Chinese Holstein dairy cows were allocated into 4 groups as a 2×2 factorial randomized complete block arrangement for 12 cows per group. Cows were fed with basal diet with 2% soybean oil (control), or basal diet plus 3.04 g of selenized yeast/d (contain 13.6 mg of selenium/d) (SS), or basal diet plus 40 g of vitamin E/d (contain 20000 IU ofα-tocopheryl/d) (VE), or basal diet plus 3.04 g of selenized yeast /d (contain 13.6 mg of selenium/d) plus 40 g of vitamin E/d (contain 20000IU ofα-tocopheryl/d) (VESS). Dry matter intake, milk yield, and percentage and yield of milk fat, protein, lactose were not significantly different among treatments. Supplementation of sodium selenite and vitamin E did not singnificantly effected the almostly all fatty acids of plasma and milk. Supplementation of sodium selenite was significantly increased the percentage of DHA (Docosahexaenoic acid) (P < 0.05) and EPA (Eicosapentaenoic acid) in plasma fatty acids. TheΔ9-desaturase index did not significantly changed among treatment. Theα-tocopherol content of milk was tended to elevant follow the supplementation of vitamin E alone and combination sodium selenite (P=0.05). Theα-tocopherol content of plasma was also increased follow the supplementation of vitamin E alone and combination sodium selenite, but it was not statistically different. The selenium content of plasma and milk were significantly inreased follow the dietry sodium selenite alone and combination vitamin E addition (P<0.01). The activity of SOD ( Superoxide dismutase) in milk was tended to elevant follow the supplementation of sodium selenite (P=0.06). The activity of GSH-Px (Glutathione peroxi-dase) in plasma was increased when sodium selenite and vitamin E alone or combination addition. but it was not statistically different.The content of MDA (Malondialdehyde) in plasma and milk did not affect when sodium selenite and vitamin E alone or combination addition. The quality of animal production had been elevanted when increasing the percentage of DHA, EPA in plasma fatty acids. Increasing special fatty acids with biological function in human diet is benefit to human health. The selenium andα-tocopherol concentration of milk and plasma in our study would partly satisfy the need of selenium andα-tocopherol for human by consuming dairy milk and meat. Increasing activity antioxidation of milk and plasma could benefet to protect the unsaturated fatty acids. The higher level vitamin E and selenized yeast of diet had not the affect infaust for dairy cows health.ExperimentⅢ:Effects of diffirent form selenium on Milk and Plasma Fatty Acid Profiles and Activity of Antioxidation in Dairy Cows Fed Fat DietsThirty six Chinese Holstein dairy cows were allocated into 4 groups as a 2×2 factorial randomized complete block arrangement for 12 cows per group. Cows were fed with basal diet with 2% soybean oil (control), or basal diet plus 3.04 g of selenized yeast/d (contain 13.6 mg of selenium/d) (SS), or basal diet plus 13.6 g of vitamin E/d (contain 13.6 mg of selenium/d) (SY). Dry matter intake, milk yield, and percentage and yield of milk fat, protein, lactose were not significantly different among treatments. The fatty acids of plasma and milk had biger affect by supplementation of selenized yeast than sodium selenite. Supplementation of selenized yeast was significantly increased the percentage of DHA (Docosahexaenoic acid) (P<0.05), EPA (Eicosapentaenoic acid) in plasma fatty acids and the percentage of cis-9, trans-11 CLA (Conjugated linoleic acid) (P<0.001), PUFA (Polyunsaturated fatty acids) (P<0.05) in milk fatty acids. TheΔ9-desaturase index did not significantly changed among treatment. The selenium content of plasma and milk and the activity of SOD ( Superoxide dismutase) in milk were significantly increased follow the dietry sodium selenite and selenized yeast addition. While the activity of SOD in plasma was not significantly inreased follow the dietry sodium selenite and selenized yeast addition. The activity of GSH-Px (Glutathione peroxi-dase) in plasma was higher increased supplementation of selenized yeast than sodium selenite. but it was not statistically different. The content ofα-tocopherol and MDA (Malondialdehyde) in plasma and milk did not significantly affect when sodium selenite and selenized yeast addition. The quality of animal production had been elevanted when increasing the percentage of DHA, EPA in plasma fatty acids and the percentage of cis-9, trans-11 CLA, PUFA in milk fatty acids. Increasing special fatty acids with biological function in human diet is benefit to human health. The selenium concentration of milk and plasma in our study would partly satisfy the need of selenium for human by consuming dairy milk and meat. Increasing activity antioxidation of milk and plasma could benefet to protect the unsaturated fatty acids.The higher level sodium selenite and selenized yeast of diet had not the affect infaust for dairy cows health.According to three experiment above, my conclusion were: 1). The dry matter intake, milk yield, and percentage and yield of milk fat, protein, lactose were not significantly different supplementation diet contain 2% soy oil with vitamin E, selenized yeast, .sodium selenite alone or vitamin E combination different form selenium. 2). Supplementation of selenized yeast than sodium selenite and vitamin E had biger affect for fatty acids of plasma and milk. Supplementation of selenized yeast was significantly increased the percentage of DHA, EPA in plasma fatty acids and the percentage of cis-9, trans-11 CLA, PUFA in milk fatty acids. TheΔ9-desaturase index did not significantly changed among treatment. 3). Supplementation of vitamin E was increase the content ofα-tocopherol of milk and slightly elevant the content ofα-tocopherol of plasma. The content of selenium in plasma and milk did not significantly affected by supplementation of vitamin E. The content of selenium in plasma and milk was significantly increased the diet supplementation of different form selenium. The efficiency was higher supplementation of selenized yeast than sodium selenite. While the content ofα-tocopherol in plasma and milk did not significantly affected the diet supplementation of different form selenium. The activity of SOD in milk was increased follow the dietry supplementation of different form selenium with the same efficiency. The activity of GSH-Px in plasma had slightly elevante follow the dietry supplementation of different form selenium. While the activity of GSH-Px in milk was not different. 4). The quality of animal production had been elevanted when increasing the percentage of DHA, EPA in plasma fatty acids and the percentage of cis-9, trans-11 CLA, PUFA in milk fatty acids. Increasing special fatty acids with biological function in human diet is benefit to human health. The selenium andα-tocopherol concentration of milk and plasma in our study would partly satisfy the need of selenium andα-tocopherol for human by consuming dairy milk and meat. Increasing activity antioxidation of milk and plasma could benefet to protect the unsaturated fatty acids. 5). The higher level vitamin E, selenized yeast and sodium selenite of diet had not the affect infaust for dairy cows health.