| Mycorrhizae fungi is an important part of ecosystem, it distributes widely, and affects the growth, physiology and zoology of host plants. Mycorrhizae fungi also can accommodate the competition relationship between different kinds of plants and circulation of ecosystem. Leymus chinensis is one of dominant species in the Songnen Plains. It has high output and it is quite suitable for feeding. Kalimeris integrifolia is an important kind of plants associated with L. chinensis. They can form different communities. Our experiment did primary investigation and analysis on species and quantity of mycorrhizae fungi living in L. chinensis– K. integrifolia community. The experimental time is from spring to autumn in 2006 and 2007. A new kind of staining techniques - Ink and Vinegar was introduced and ameliorated. The purpose is to find out the distribution and the function of Mycorrhizae fungi in the Songnen Plains. The results as follows:Our experiment ameliorated a new kind of staining techniques - ink and vinegar. Ten kinds of domestic ink were contrasted, and different combinations of KOH clearing time, staining time and fading time were attempted. At last, the best combination was found: for L. chinensis, Yingkesi 9930 ink or Yingxiong 203 ink, 10% KOH clearing 10min, staining 5min and fading 40min; for K. integrifolia, Yingkesi 9930 ink, KOH clearing 5min, staining 5min and fading 30 min.In L. chinensis– K. integrifolia community, there is very obvious changes about the species and quantity of spores and fungi. The quantity of spores in L. chinensis community (42741/100kg dry soil) is higher than it in L. chinensis + K. integrifolia community (38999/100kg dry soil), the quantity of spores in L. chinensis community is the lowest (34887/100kg dry soil). The mycorrhizal fungi colonization of roots is higher in K. integrifolia community (63%) than it is in L. chinensis + K. integrifolia community (50%; 47%); the colonization of roots is the lowest in L. chinensis community (40%). The reason may be the speciality of arbuscular mycorrhizal, and the diversity of plants. From spring to summer, the quantity of spores in L. chinensis– K. integrifolia community was reducing, from summer to autumn, the quantity of spores in– K. integrifolia community was increasing; the arbuscular mycorrhizal colonization of roots changed a lot, it can be attributed to the change of the seasons and the different kinds of plants in the community.The species and quantity of mycorrhizal fungi in L. chinensis– K. integrifolia community changed obviously when the species of host plants changed and the season passed. The further researches on the mechanisms for influencing mycorrhizal fungi are expected. |
