Effects Of Insulin And Casein Hydrolysate On The Liver Growth And Development Of IUGR Pigs

In order to investigate the effects of insulin and casein hydrolysate on the growth and development of liver in intrauterine growth retardation (IUGR) piglets, the experiment compared the difference of liver weight, content of protein, DNA and RNA, activities of superoxide dismutase(SOD), hepatic lipase(LP), glutamine-oxaloacetic transaminase(GOT), glutamic-pyruvic transaminase(GPT), contents of free fatty acid(FFA), malonddialdehyde (MDA), total cholesterol(TC), triglyceride (TG), insulin, insulin-growth factor I(IGF-I) in plasma and activities of SOD, HL, catalase(CAT), glutathione peroxidase(GSH-Px), malate dehydrogenase(MDH), contents of glycogen, MDA in liver among newborn piglets, piglets bottle-fed for 3 days with cow-milk and cow milk supplemented with insulin, casein or casein hydrolysate.In the first experiment, fifteen Sutai IUGR piglets were selected from five litters, three piglets from every litter, and divided into three groups: newborn group(N), milk group(M) and milk supplemented with insulin (60IU/L) group(I), five IUGR piglets in every group. Compared with group A, liver weight and glycose in plasma of IUGR piglets bottle-fed for 3 days was increased significantly(P<0.05), the content of protein, DNA and RNA was increased (P<0.05). The content of TG, TC, MDH was significant increasedtotal proteolytic activity of protease in stomach but total milk-clotting activity of gastric protease was decreased(P<0.01), while FFA in plasma was decreased(P<0.01). Content of MDA of IUGR piglets in group I was decreased and the activities of SOD, GSH-Px, CAT, GOT, GPT was increased significant(P<0.05). The content of insulin in plasma of IUGR piglets bottle-fed for 3 days was in a high level(P<0.01).The results show that oral insulin can stimulate the growth of liver of the IUGR piglets and the maturity of function.In the second experiment, fifteen Sutai piglets were selected from five litters, three IUGR piglets from every litter, and allotted to three groups: newborn group(A), casein group(C) and casein hydrolysate group(CH), five IUGR piglets in every group. The piglets in group C and CH were fed milk substituted 1.5% casein and 1.5% hydrolysed casein solution for 10% (v/v) milk for 3 days. After the piglets were fed artificially for 3 days, the result showed in this experiment is similar to experiment one. Although the body weight was decreased, the liver weight of group C and group CH was increased quickly ( P>0.05 ), while the content of DNA in the liver in IUGR piglets of group CH was increased significantly (P<0.05) , and the content of RNA and protein was increased rapidly at the same time (P>0.05) . The activities of SOD, CAT and GPT were increased obviously ( P<0.05 ), in contrast, the content of MDA decreased (P<0.05). After the piglets were fed manually for 3 days, the level of FEA in plasma was decreased significantly, and the level of the MDH in the liver and the TG in plasma was risen up (P<0.05 ), but the level of TC and HL kept alike. The content of IGF-I and insulin in three groups had no significant changes ( P>0.05 ). The data indicated that the casein and casein hydrolysate could promote the growth and development of the liver in IUGR piglets, also they could enhance the capability of the antioxidant and the metabolism of glycose and grease in the liver of IUGR piglets.