Effect Of Different Hormone And Moist-Chilling On Seed Germination And Seedling Growth Of Fengdan

Tree peony is one of the traditional famous flower. At present, it is engrafted in orderto propagation, and Paeonia ostii seedling is one of the most importemt bickiron. But, itsepicotyl beared dormancy trait which restrict the yield of seedling. It was reported thathormones and moist chilling can breack the dormancy of epicotyl dormancy. But thephysiological mechanism have not been found. The epicotyl modality variety with the rootsdevelopment; the effect of several hormones and moist-chilling treatments on breakingepicotyl dormancy, content change of nutrient in the relieving period, and seedling growthstatus of peony were studied. Main results:The epicotyl modality variety with the roots development and content change ofnutrient: The degree of differentiation of epicotyl was correlated with the root length ofseeding; The epicotyl growth increment that was in need of perception of low-temperaturewas achieved while the root length of seeding was more than 4cm. Before breaking epicotyldormancy, a small amount of nutrient was needed. The content of three kinds of protein wasstable while the content of soluble sugar and starch demonstrated the tendency of goingdown.Effect of different content of GA₃, 6-BA, IBA on epicotyl germination: The 4pretreatments effectively broke the epicotyl dormancy of peony seeds which root waslonger than 4cm. However, GA₃ and moist chilling treatments were the most effective,6-BA ranked the next, and IBA was the worst. Seed germination rate was the highest at300mg·L^-1 concentration of GA₃; The germination rate increased gradually with theprolonging of moist chilling treatment time, and the rate was highest on the 32nd day. Seedgermination rate was the highest in 100 mg·L^-1 concentration of IBA and 6-BA, and itdecreased with the increase of the concentration. Seed germination was fast with GA₃treatment, but the time of emergencing regular seeding was long; While seed germinationwas slow, but the time of emergencing regular seeding was short.Effect of different content of GA₃, 6-BA, IBA and moist chilling on nutrient contentand the epicotyl modality variety: The contents of albumin, gloublin, and glutenin all risedfirstly then dropped. The maximum appeared at different time, of which the peek value ofalbumin appeared later. Among these treatments, the contents of three kinds of protein werethe highest with GA₃ treatment; The content of protein was also influenced by theconcentrations of hormones. It showed good effect with high concentration of 6-BA and low concentration of IBA. 300mg·L^-1 concentration obtained the best effect in the GA₃treatment. The longer low-temperature time was given as better the effect was.The growth of leaves was affected significantly by 6-BA, IBA, and low-temperaturetreatments. Leaf area, leaf length, leaf width, and leaf cycle length all increasedsignificantly. GA₃ had almost no effect on leaf area. The change laws of WRS, root length,root volume, the amount of root tips, and biomass accumulation were in accordance, andthe results were all higher than control groups. It showed good effect with highconcentration of 6-BA and low concentration of IBA. 300mg·L^-1 concentration obtained thebest effect in the GA₃ treatment. It appeared a tendency to first increased and thendecreased with low-temperature treatment.The net photosynthetic rate and stoma conductance decreased while the water useefficiency was improved of peony seeding with GA₃ and low-temperature treatments. Theminimum of net photosynthetic rate and stoma conductance was under 300mg·L-1concentration among different GA₃ treatments, and there were no significant changesamong different low-temperature treatments. Kinds of index of 6-BA and GA₃ treatmentshad no significant difference with that in control group. The parameter of seeding was notchanged significantly by those four treatments.