Selection And Identification Of Transgenic MTI2 And Bt Arabidopsis Thaliana

The selection and identification of transgenic plants were important in the course of transgene breeding research. The progenies of the transgenic Arabidopsis thaliana carrying with MTI2 gene were selected and identification with resistance phenotypic marker and polymerase chain reaction (PCR) analysis from T1 to T5 generation in this thesis, and the Kanamycin-resistant plants were regenerated. The transgenic Bt plants and transgenic MTI2 plants were tested in laboratory bioassays and compared Bt gene with MTI2 gene for activity against the larvae of P.xylostella. Subsequently, the effect of backcrossing times on P.xylostella resistance of transgenic Bt Arabidopsis were discussed. The main results as follows:1. 100 pure transgenic lines was obtained after kanamycin-resist selection and PCR detection in the T5 generation.2. The study demonstrated that transgenic plants with MTI2 gene no remarkable mortality was observed although a delay in larval development did occur. Contrarily, introduction of a Bt gene into Arabidopsis offered high levels of protection against P.xylostella infestation. So this gene can be a good candidate for engineering of transgene.3. The result of analysis backcrossing progeny shows that the resistance to P. xylostella of transgenic Bt plants was reduced as the increase of backcrossing times. According to this study, 1-2times backcrossing could greatly improve the resistance P. xylostella.