AFLP And SSR Analysis Of Pollinated Generation Between Carya Cathayensis And C.illinoinensis

Carya Nutt is an important oil tree and dry fruit in the world.Because of its large economic value,it is significant to improve the income of montane farmers.The taste of Carya cathayensis which has abundant nutrition is faint sweet.And C.illinoinensis which has big nut,thin shell,high kernel rate is easy to rind and get the kernel. Hybrids which had better phenotype and quality was acquired through hybridization between C.cathayensis and C.illinoinensis.It provided new theoretic base for fine variety breeding and was of great theoretic and practical importance to improve C.cathayensis to study the molecular marker of the pollinated generations. The F1 pollinated colony between C.cathayensis and C. illinoinensis was constructed through covering bag. The genetic mutation of F1 generations were analyzed using AFLP and SSR.The main results were summaried as follows:(1) The character between pollinated fruits and antitheses had marked diversity. The influence of controlled pollination was large to phenotype. And pollinated fruits was better than antitheses in per nut weight, nut length, nut width, nut shape index, nut shell thinkness, shell weight and kernel weight.(2) The methods of improved CTAB was better to extract genomic DNA of the F1 pollinated colony between C.cathayensis and C. illinoinensis.The concentration and purity of DNA accorded to the needs of PCR and cutting.(3) The effect of cutting 6h or over night in cutting and joining at one time system and cutting 2h in first cutting and then joining system to AFLP selective amplification was same.Because of complexity of first cutting and then joining system, cutting 6h or over night in cutting and joining at one time system was always used. It had no effect to AFLP selective amplification in diluted multiple of cutting and joining produces and pre-amplification produces.(4) The optimal SSR reaction system of F1 generation was 30μl of total volume containing 1×PCR Buffer, 2.0 unite of Taq DNA polymerase, 0.20 mmol·L-1 of dNTP, 1.5 mmol·L-1 of Mg2+ ,600ng of template DNA and 0.13μmol·L-1 of each primer.(5) The genetic mutation of F1 generation between C.cathayensis and C. illinoinensis was analyzed using 10 pairs AFLP primers. The results indicated that there are not different between female parent and its generations, but difference between male parent and its generations with each primers.(6) The genetic mutation of F1 generation between C.cathayensis and C. illinoinensis was analyzed using 19 pairs SSR primers.The results indicated that there are not different between female parent and its generations with each primers, but difference between male parent and its generations in 4 primers and no difference in 9 primers.