The RAPD Analysis And Correlation Analysis On Tomato Inbred Lines And Hybrid Progenies F1

Twenty two tomato materials were adopted and their agronomic traits and quality were surveyed. CTAB was applied to extraction materials' DNA, then, the reaction of RAPD, the concentration of agent that referred to the RAPD and concentration of agarose were optimized. The results are as follows:1. The improved CTAB was applied to extract DNA. The ratio of OD260/OD280 was from 1.8 to 2.0 and OD260/OD230 was more than 2. Furthermore, the results of electrophoresis was clear. All above indicated that the DNA was qualified for RAPD analysis.2. Orthogonal experiment design was used for setting up optimized RAPD reaction. Each sample was amplified in a reaction mixture (25μL in total volume) containing 40ng template DNA, 200μmol/L, deoxynucleoside triphosphate (SBS), 1.25U of Taq polymerase, 5μL of 10×PCR buffer, 1.5μL of MgCl₂ and 14μL of ddH₂O. The reaction mixture was cycled through the following temperature profiles: 94℃for 5min, 94℃for 30s (predenaturation), 36℃for 30s, and 72℃for 1min for 40 cycles. The PCR was terminated at 72℃for 7min.3. Both concentrations 1.6% and 2.0% of agarose can distinguish various DNA, but 1.6% was adopted for the sake of cost.4. 11 primers were screened out of 40 primers. The 11 primers amplified 74 DNA fragments and each primer can amplify 6.67 fragments. There were 61 polymorphic fragments among total 74 fragments, that accounted for 82.4% and each primer could amplify 5.1 polymorphic fragments average.5. The cluster analysis and relationship analysis were carried out and the results showed that the hybrid progenies F1 inclined to male inbred lines in heredity. The correlation analysis was performed between the genetic distance and plant height, mean fruit weight and total soluble solid, but the correlation coefficient was not significant, so the work of heterosis predicted by RAPD should be studied further.