On Research Of The Relevance Between The Change In Cell Structure Of Tobacco Leaf And Enzyme Activity And Different Maturity Of The Flue-cured Tobacco

The colour of main nerve in the flue-cured tobacco leaf turns green into white,the angle number of degree in the stem leaf increases, these are main changes ofexterior characteristic in the mature process of the flue-cured tobacco leaf. This kindof change is in close relation to maturitis of the flue-cured tobacco leaf. At present, inthe target of determining maturity of the flue-cured tobacco leaf, the leaf nervebleached and the stem leaf angle increase, but there are only concise description, so itis a bit more difficult for the practice participants to grasp. The research of rule on theleaf nerve bleaching in the mature process of the flue-cured tobacco leaf, the researchof mechanism on the stem leaf angle number of degree increasing, those which willprovide the corresponding theory basis to determine the maturity target of theflue-cured tobacco and to enhance the accuracy of determining the maturity target ofthe flue-cured tobacco. All these are expected to provide certain technical support inthe actual production of instructing mature harvest of the flue-cured tobacco leaf.This article takes the flue-cured tobacco variety K326, Honghuadajinyuan, G80as material cultivated in the experiment field in 2006, conducted a series of researchand analysing on those change situation in chlorophyll,chloroplast and POD in themature process of the flue-cured tobacco leaf, on those change situation in anglenumber of degree of the leaf leaf, on those differentiation situation in abscission zonecell.Main findings are as follows:1. Conducts the track research to the flue-cured tobacco leaf main nervechloroplast density and the chlorophyll content in themature process change,discovered that there is obvious difference in the different maturity the flue-curedtobacco leaf main nerve chloroplast density and the chlorophy content whichincreases along with the reduction of the flue-cured tobacco leaf maturity.2. Uses the paraffin section movie-making law to observe the flue-cured tobaccopetiole stem leaf living place cell in mature process change, and has conducted theresearch to the different maturityflue-cured tobacco leaf stem leaf angle numberofdegree, discovered that the area appears in the mature process of flue-cured tobacco petiole stem leaf living place cell; The synchronization area differentiation and theangle number of degree of the flue-cured tobacco leaf stem leaf conduct at the sametime; The stem leaf angle number of degree increases along with the leaf maturity;The angle number of degree increased range peak value in different variety of theflue-cured tobacco leaf stem leaf appears to be in different time.3. Discovered through determination in the flue-cured tobacco petiole PODactivity: the POD activity rises before the flue-cured tobacco leaf is ripe, the PODactivity drops after the flue-cured tobacco leaf is ripe, living place will tally withpetiole stem leaf living area cell differentiation peak. Topping has differentinfluence on the flue-cured tobacco variety POD activity.4. Carried on the analysis to the flue-cured tobacco mature periodleaf stockadecell density change and the cell intrinsic chemical composition relevance, finallydemonstrated K326 and Honghuadajinyuan leaf's water-solubility totalsugar contentand the stockade cell number is extremely positive relevance; The G80 leaf's nicotineand the stockade cell number is negative relevance, K326 and Honghuadajinyuanleaf's nicotine content and the stockade cell number is extremely negative relevance.