The Effect Of RRS On Microbe Communities Diversity And Nitrogen Transition In Rhizosphere Soil

The research took six different genotype soybeans samples to analysis. It studied the specialfunctional microbe communities on the ecology of transgenic soybeans in the black soil (there weresome microbe communities related to the nitrogen transform, such as bacteria, rhizobia bacteria,ammonifying bacteria, nitrifying bacteria) and the biochemical processes (ammonifying intensity,nitrifying bacteria), the enzyme dynamic changes of RRS in rhizosphere soil. It also analysed theeffect of RRS to the microbial diversity in rhizosphere soil with modem molecular biologymethods (PCR-DGGE). Therefore our objective was to identify the effect of RRS on bacteriumcommunities, the effect on the ammonification and the nitrification intensity in the rhizosphere soil.The aims of the study were to supply the effect of transgenic soybean on ecology systemIn the Bearpod for period, the bacteria quantities of RRS achieved the most in rhizosphere soil.The mean value of RRS was lower than RRS-S and other genotype soybeans. The fungi quantitiesof RRS were lower than RRS-S and other genotype soybeans in the whole periods. Theactinomycete quantities of different genotype soybeans achieved significant level in the wholeperiods. The actinomycete quantities of RRS were lower than other genotype soybeans. It mightprove that RRS would decrease the quantities of actinomycete.The change trend of different genotype soybeans was consistent in the whole growing stages.The ammonifying bacteria quantities of different genotype soybeans achieved remarkale. Theammonifying bacteria quantities of RRS were lower than RRS-S, D-46 and H-101. In the wholegrowing period, the nitrifying bacteria quantities of RRS were lower than RRS-S.The effect of rhizobium bacterie quantities on different genotype soybeans in rhizosphere soilwas significantly.The rhizobium bacteria quantities of RRS was lower than RRS-S.The root nodulequantities of RRS were lower than that other genotype soybeans in rhizosphere soil.There were remarkable levels of biochemical role in the rhizosphere soil (ammonifyingintensity, nitrifying intensity). The ammonifying intensity and the quantities of ammonifyingbacteria had the good correlation in inflorescence period and bear pods for period. There were goodcorrelation between nitrifying bacteria and nitrifying intensity in these periods.The paper mainly determined urease and catalase activities in rhizosphere soil. In the entiregrowth period, the urease activity of RRS was lower than RRS-S in rhizosphere soil. There was nosignificant impact of different genotype soybeans on catalase activities. In inflorescence stage, the soil bacteria diversity index and homogeneous degrees index ofRRS are 1.518 and 0.643. In bear pods for period, the soil bacteria diversity index andhomogeneous degrees index of RRS in rhizosphere soil were 1.865, 0.741. In the drum is each, thesoil bacteria diversity index and homogeneous degrees index of RRS in rhizosphere soil were 1.432and 0581. The soil bacteria diversity index and homogeneous degrees index of RRS were lowerthan RRS-S and Y-S. There were consistent between DGGE analysis of the results and theconventional analysis of the conclusions.Genetically, the soil ammonia-oxidizing bacteria diversity index and homogeneous degreesindex of to RRS in the rhizosphere soil were lower than RRS-S and Y-S.