Determination Of Bursin Content In Layers' Blood And Part Of Organs

This experiment established a method,High Performance Liquid ChromatograpHy to separate and analysis bursin from layers' blood and organs.With this method,the rise and fall regularity of bursin content with days' increasing has been found.The study selected one-day-old Roman chickens and randomly divided them into 2 groups,groupⅠand groupⅡ.GroupⅠcontained 330 chickens and groupⅡcontained 30 chickens.The two groups were seperately abataged group and benificed for blood group.Chickens in groupⅠwere abataged for blood,bursa,spiced and spinal cord,and there were 29 duplications test from 1st day to 26th week.At the same time,chickens in groupⅡwere benificed for blood.After high speed freezing ccntrifugalization, etc,HPLC with ultraviolet ray detection was used to detect bursin in chickens' blood and tissues.Detections conditions of HPLC were the following:mobile phase components with volume proportion were ultrapure water:acetonitrile:TFA=80:20: 0.1,speed of mobile phase is 1.0 mL/min,temperature of the column is 25℃,the detection wavelength is 215nm,the quantity of injection is 10ul.The verification experiment indicated that the detection method established has good reproducibility and high precision,and the apparatus function is stable.Bursin was found in layers's blood,bursa,spleen and spinal cord,but bursin content in blood and erery tissue was different.With days increasing,the values of bursin content in bursa,blood and spleen were all small firstly,then large,and little at last.In bursa,the minimum value of bursin content was 0.2836 mg/mL when the chickens were one day old,and the maximal value was 1.6808 mg/mL when the layerss were 8 weeks old.The value was more than 1.60 mg/mL from 52nd day to 10th week,then the value began to become little quickly.From 18th week on,the value was less than that of blood.After 19th week,the descent speed began to become little gradually and it began to be stable but little after 23rd week.The stable value was larger than 0.37 mg/mL but less than 0.40 mg/mL,and it was larger than that of week 1 but less than that of week 2.Early days,the value of bursin content in blood was less than that of bursa and the change tendency in blood was similar to that in bursa.The value's descent speed was less than that of bursa after 12th week.From 18th week on,it was larger than that of bursa and it was larger than 0.51 mg/mL but less than 0.54 mg/mL after 23rd week.And it was larger than that of bursa at the same term.The change tendency of bursin content in spleen was consistent with that of blood but the value was less than that of blood.In spinal cord,the bursin content value's change tendency was different from those of bursa and blood. Correspondingly,the value was little before 12th week and after 12th week,the values kept large.But the value was little throughout.Results of the correlation analysis showed that the change tendency of bursin in blood had close relation with that in bursa before 12th week and bursin in blood had close relation with bursa.From 12th week,the bursa began to degenerate gradually and bursin in blood had clode relation with spinal cord.We can confer that other organs that can produce bursin resist in layers' organism after the bursa's deneneration, and spinal cord maybe one kind of them.we also confer that bursin in spleen is from bloood and spleen itself can't produce bursin.This study found the change tendencies of bursin content in chickens' blood and tissues and make it the base of study to bursin application and the relation between bursin and disease infection.